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Product Info Summary
| SKU: | PROTP01137-10 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | HEK293 cell |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant TGF beta 1 (Transforming growth factor beta 1) protein, AF
View all TGFB1 recombinant proteins
SKU/Catalog Number
PROTP01137-10
Size
5ug,20ug,100ug
Tag
His Tag (N-term)
Description
The transforming Growth Factors beta (TGF beta) family of cytokines are ubiquitous, multifunctional, and essential to survival. They play the central roles in growth, development, inflammation, repair, and host immunity. The mammalian TGF beta isoforms (TGF beta 1, beta 2 and beta 3) are secreted as potential precursors and possess a variety of cell surface receptors and produces at least two mediate signal transductions.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant TGF beta 1 (Transforming growth factor beta 1) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP01137-10)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution of PBS, pH 7.0. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
44.341kDa
Molecular weight
The protein has a calculated MW of 13.7 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human TGF beta 1 is approximately >5 x 10⁷ IU/mg. Measure by its ability to induce proliferation in MCF-7 cells. The ED₅₀ for this effect is <3.2 ng/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
A DNA sequence encoding Human CD28 ECD (# P01137) (Met1-Ser390) was expressed with polyhistidine tag at the N-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile 10 mM HCl to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human TGF beta 1
Protein Target Info & Infographic
Gene/Protein Information For TGFB1 (Source: Uniprot.org, NCBI)
Gene Name
TGFB1
Full Name
Transforming growth factor beta-1 proprotein
Weight
44.341kDa
Superfamily
TGF-beta family
Alternative Names
Differentiation inhibiting factor, Cartilage-inducing factor, CED, DPD1, IBDIMDE, LAP, TGFB TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TGFB1, check out the TGFB1 Infographic
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In this infographic, you will see the following information for TGFB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant TGF beta 1 (Transforming growth factor beta 1) protein, AF (PROTP01137-10)
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