Human recombinant Galectin-1 protein, AF

Galectin-1 protein, Human

Galectin-1 (Gal-1) is a lectin family member. Galectin-1 contains one carbohydrate recognition domain (CRD), responsible for β-galactoside binding, and is biologically active as homodimers. Several binding partners for galectin-1 have been identified, including CD45, CD43, CD69, pre-BCR, and vascular endothelial Growth Factors R2. Galectin-1 is constitutively presented in the immune system and other tissues, contributing to cell growth, proliferation, and apoptosis. The galectin-1 plays a central role in regulating acute and chronic inflammatory responses.

Product Info Summary

SKU: PROTP09382-3
Size: 5ug,20ug,100ug,500ug,1mg
Origin Species: Human
Source: Escherichia coli
Application: Cell Culture

Product Name

Human recombinant Galectin-1 protein, AF

View all Galectin-1 recombinant proteins

SKU/Catalog Number

PROTP09382-3

Size

5ug,20ug,100ug,500ug,1mg

Tag

His Tag (N-term)

Description

Galectin-1 (Gal-1) is a lectin family member. Galectin-1 contains one carbohydrate recognition domain (CRD), responsible for β-galactoside binding, and is biologically active as homodimers. Several binding partners for galectin-1 have been identified, including CD45, CD43, CD69, pre-BCR, and vascular endothelial Growth Factors R2. Galectin-1 is constitutively presented in the immune system and other tissues, contributing to cell growth, proliferation, and apoptosis. The galectin-1 plays a central role in regulating acute and chronic inflammatory responses.

Storage & Handling

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Cite This Product

Human recombinant Galectin-1 protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP09382-3)

Form

Lyophilized

Formulation

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Purity

>98% as determined by SDS-PAGE.

Predicted MW

14.716kDa

Molecular weight

The protein has a calculated MW of 15.5 kDa. The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).

Activity

Measured by its ability to agglutinate human red blood cells. The ED₅₀ for this effect is <2 μg/mL.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Amino Acid Sequence

ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD with polyhistidine tag at the N-terminus.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Validation Images & Assay Conditions

Gene/Protein Information For LGALS1 (Source: Uniprot.org, NCBI)

Gene Name

LGALS1

Full Name

Galectin-1

Weight

14.716kDa

Alternative Names

Beta-galactoside-binding lectin L-14-I, Galaptin, 14 kDa lectin, S-LAC lectin-1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on LGALS1, check out the LGALS1 Infographic

LGALS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LGALS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $77

SKU:PROTP09382-3

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