Product Info Summary
SKU: | PROTP09382-3 |
---|---|
Size: | 5ug,20ug,100ug,500ug,1mg |
Origin Species: | Human |
Source: | Escherichia coli |
Application: | Cell Culture |
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Product info
Product Name
Human recombinant Galectin-1 protein, AF
View all Galectin-1 recombinant proteins
SKU/Catalog Number
PROTP09382-3
Size
5ug,20ug,100ug,500ug,1mg
Tag
His Tag (N-term)
Description
Galectin-1 (Gal-1) is a lectin family member. Galectin-1 contains one carbohydrate recognition domain (CRD), responsible for β-galactoside binding, and is biologically active as homodimers. Several binding partners for galectin-1 have been identified, including CD45, CD43, CD69, pre-BCR, and vascular endothelial Growth Factors R2. Galectin-1 is constitutively presented in the immune system and other tissues, contributing to cell growth, proliferation, and apoptosis. The galectin-1 plays a central role in regulating acute and chronic inflammatory responses.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant Galectin-1 protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP09382-3)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
14.716kDa
Molecular weight
The protein has a calculated MW of 15.5 kDa. The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measured by its ability to agglutinate human red blood cells. The ED₅₀ for this effect is <2 μg/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD with polyhistidine tag at the N-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
Click image to see more details
SDS- PAGE analysis of recombinant human Galectin-1
Protein Target Info & Infographic
Gene/Protein Information For LGALS1 (Source: Uniprot.org, NCBI)
Gene Name
LGALS1
Full Name
Galectin-1
Weight
14.716kDa
Alternative Names
Beta-galactoside-binding lectin L-14-I, Galaptin, 14 kDa lectin, S-LAC lectin-1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on LGALS1, check out the LGALS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LGALS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant Galectin-1 protein, AF (PROTP09382-3)
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