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Product Info Summary
| SKU: | PA1945 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband®
SKU/Catalog Number
PA1945
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Milk Fat Globule 1/MFGE8 Antibody catalog # PA1945. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1945)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Human Milk Fat Globule 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1945 is reactive to MFGE8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
43123 MW
Background of MFG-E8
MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al. (1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al. (2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al. (2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1945 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human milk 5 μl, human milk 25 μl
IHC: human placenta tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MFGE8 using anti-MFGE8 antibody (PA1945).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human milk 5 μl,
Lane 2: human milk 2.5 μl.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFGE8 antigen affinity purified polyclonal antibody (Catalog # PA1945) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFGE8 at approximately 43 kDa. The expected band size for MFGE8 is at 43 kDa.
Click image to see more details
Figure 2. IHC analysis of MFGE8 using anti-MFGE8 antibody (PA1945).
MFGE8 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MFGE8 Antibody (PA1945) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MFGE8 (Source: Uniprot.org, NCBI)
Gene Name
MFGE8
Full Name
Lactadherin
Weight
43123 MW
Alternative Names
Lactadherin;Breast epithelial antigen BA46;HMFG;MFGM;Milk fat globule-EGF factor 8;MFG-E8;SED1;Lactadherin short form;Medin;MFGE8; MFGE8 BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47 milk fat globule EGF and factor V/VIII domain containing lactadherin|O-acetyl disialoganglioside synthase|breast epithelial BA46|medin|milk fat globule-EGF factor 8 protein|sperm associated 10|sperm surface protein hP47
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MFGE8, check out the MFGE8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MFGE8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband® (PA1945)
Hello CJ!
PA1945 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Innate Immune Factors in Mothers' Breast Milk and Their Lack of Association With Rotavirus Vaccine Immunogenicity in Nicaraguan Infants.
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Customer Q&As
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3 Customer Q&As for Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband®
Question
We are currently using anti-Human Milk Fat Globule 1/MFGE8 antibody PA1945 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The anti-Human Milk Fat Globule 1/MFGE8 antibody (PA1945) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-05
Question
I see that the anti-Human Milk Fat Globule 1/MFGE8 antibody PA1945 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary carcinoma mammary gland using anti-Human Milk Fat Globule 1/MFGE8 antibody PA1945. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-22
