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Product Info Summary
| SKU: | EK0480 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. |
Product info
Product Name
Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®
View all Osteoprotegerin/TNFRSF11B ELISA kits
SKU/Catalog Number
EK0480
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF11B in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0480)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E22-L401
Sensitivity
<5 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0480 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0480 is reactive to TNFRSF11B in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
Application Guarantee
EK0480 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Osteoprotegerin/TNFRSF11B
Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system. The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0480-CAP | Anti-Human TNFRSF11B Pre-coated 96-well strip microplate | 1 |
| EK0480-ST | Human TNFRSF11B Standard | 2 vials, 10 ng/tube |
| EK0480-DA | Human TNFRSF11B Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.002 | 0.081 | 0.163 | 0.282 | 0.550 | 0.841 | 1.506 | 2.019 |
Data Example Images
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Human OPG PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Osteoprotegerin/TNFRSF11B, Dilution ratio of 1:1, concentration in serum and plasma is around 50 pg/ml..
Some articles we found to cite concentrations of Osteoprotegerin/TNFRSF11B in samples: 24845712 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 132 | 650 | 2696 | 130 | 692 | 2678 |
| Standard deviation | 10.42 | 37.7 | 113.23 | 11.57 | 51.2 | 136.57 |
| CV (%) | 7.9% | 5.8% | 4.2% | 8.9% | 7.4% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Osteoprotegerin/TNFRSF11B in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 132 | 142 | 137 | 133 | 136 | 3.93 | 2.8% |
| Sample 2 | 650 | 625 | 651 | 585 | 627 | 26.78 | 4.2% |
| Sample 3 | 2696 | 3024 | 2657 | 2825 | 2800 | 143.23 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF11B (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF11B
Full Name
Tumor necrosis factor receptor superfamily member 11B
Weight
46026 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 11B;Osteoclastogenesis inhibitory factor;Osteoprotegerin;TNFRSF11B;OCIF, OPG; TNFRSF11B OCIF, OPG, PDB5, TR1 TNF receptor superfamily member 11b tumor necrosis factor receptor superfamily member 11B|osteoclastogenesis inhibitory factor|osteoprotegerin|tumor necrosis factor receptor superfamily, member 11b
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF11B, check out the TNFRSF11B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF11B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine® (EK0480)
Hello CJ!
EK0480 has been cited in 30 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cajanolactone A from Cajanus cajan Promoted Osteoblast Differentiation in Human Bone Marrow Mesenchymal Stem Cells via Stimulating Wnt/LRP5/β-Catenin Signaling
Changes in Adipose Tissue Distribution and Association between Uric Acid and Bone Health during Menopause Transition
Loss of RUNX3 expression inhibits bone invasion of oral squamous cell carcinoma
An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells
Premature atherosclerosis in children with beta-thalassemia major: New diagnostic marker
Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis
Serum Receptor Activator of Nuclear Factor‐κ B Ligand, Osteoprotegrin, and Intact Parathyroid Hormone in Hemodialysis and Renal Transplant Patients
Coupled OPG-Fc on Decellularized Aortic Valves by EDC/NHS Attenuates Rat MSCs Calcification In Vitro
VDR, RANKL and OPG polymorphisms as possible predisposing cofactors of postmenopausal osteoporosis: explorative study in Italian population
Osteolytic lesions, cytogenetic features and bone marrow levels of cytokines and chemokines in multiple myeloma patients: Role of chemokine (C-C motif) ligand 20
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
10 Customer Q&As for Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®
Question
Does this ELISA detect OPG bound to RANKL?
Verified customer
Asked: 2022-12-14
Answer
EK0480 detects total OPG including OPG bound to RANKL.
Boster Scientific Support
Answered: 2022-12-14
Question
Is there a ‘Quick version’ available for the EK0480?
Verified customer
Asked: 2022-03-08
Answer
Currently, we do not have a 90-minute Quick ELISA kit for Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine (EK0480). However, our lab continues to develop new Quick ELISA kits every month. We will add the "Quick version" of EK0480 to our catalog when it is ready.
Boster Scientific Support
Answered: 2022-03-08
Question
Q: can you recommend the dilution ratio of serum samples for detection of Osteoprotegerin in Human saliva? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-12-13
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Osteoprotegerin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-12-13
Question
Q: is it okay to use citrate plasma as samples in Human Osteoprotegerin Picokine® ELISA Kit (Catalog # EK0480)?
Verified Customer
Verified customer
Asked: 2020-11-14
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Osteoprotegerin causing degradation of its protein structure. Osteoprotegerin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Osteoprotegerin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-11-14
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Osteoprotegerin using the EK0480 Human Osteoprotegerin Picokine® ELISA Kit?
B. Miller
Verified customer
Asked: 2019-10-18
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-10-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-28
Question
Q: What is the optimal O.D. value for Osteoprotegerin ELISA kit? I performed your Osteoprotegerin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Osteoprotegerin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Osteoprotegerin ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of Osteoprotegerin that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2018-10-03
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-26
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-26
Question
Q: if the enzyme conjugated Osteoprotegerin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Osteoprotegerin antigen?
Verified Customer
Verified customer
Asked: 2016-02-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-02-25
Question
Q: Can Osteoprotegerin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2014-02-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2014-02-11
