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Product Info Summary
| SKU: | EK1378 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1378
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine® (96 Tests). Quantitate Human NRG1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1378)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S2-K246
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1378 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1378 is reactive to NRG1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1378 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of NRG1
Neuregulin 1 or NRG1 is a protein that in humans is encoded by the NRG1 gene. It is mapped to 8p12. NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). It is also essential for the normal development of the nervous system and the heart. Furthermore, it was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1378-CAP | Anti-Human NRG1 Pre-coated 96-well strip microplate | 1 |
| EK1378-ST | Human NRG1 Standard | 2 vials, 10 ng/tube |
| EK1378-DA | Human NRG1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.080 | 0.126 | 0.171 | 0.184 | 0.312 | 0.521 | 1.018 | 1.894 |
Data Example Images
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Human Neuregulin-1/NRG1-Beta1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect NRG1, Dilution ratio of 1:10, concentration in serum and plasma is 2-40 ng/ml..
Some articles we found to cite concentrations of NRG1 in samples: 22599357 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 158 | 621 | 1900 | 159 | 607 | 1910 |
| Standard deviation | 12.16 | 30.42 | 81.7 | 14.46 | 40.06 | 95.5 |
| CV (%) | 7.7% | 4.9% | 4.3% | 9.1% | 6.6% | 5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of NRG1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 158 | 150 | 151 | 135 | 148 | 8.38 | 5.6% |
| Sample 2 | 621 | 678 | 601 | 711 | 652 | 43.92 | 6.7% |
| Sample 3 | 1900 | 1866 | 2082 | 1930 | 1944 | 82.55 | 4.2% |
Protein Target Info & Infographic
Gene/Protein Information For NRG1 (Source: Uniprot.Org, NCBI)
Gene Name
NRG1
Full Name
Pro-neuregulin-1, membrane-bound isoform
Weight
70392 MW
Superfamily
neuregulin family
Alternative Names
Pro-neuregulin-1, membrane-bound isoform;Pro-NRG1;Neuregulin-1;Acetylcholine receptor-inducing activity;ARIA;Breast cancer cell differentiation factor p45;Glial growth factor;Heregulin;HRG;Neu differentiation factor;Sensory and motor neuron-derived factor;NRG1;GGF, HGL, HRGA, NDF, SMDF; NRG1 ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF-IT2, SMDF, NRG1 neuregulin 1 pro-neuregulin-1, membrane-bound isoform|acetylcholine receptor-inducing activity|glial growth factor 2|heregulin, alpha (45kD, ERBB2 p185-activator)|neu differentiation factor|pro-NRG1|sensory and motor neuron derived factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NRG1, check out the NRG1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NRG1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine® (EK1378)
Hello CJ!
EK1378 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Novel treatment avenues for uterine leiomyoma: a new implication for endothelin?
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human NRG1 Picokine® ELISA Kit (Catalog # EK1378)?
Verified Customer
Verified customer
Asked: 2020-11-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of NRG1 causing disruption of its protein structure. NRG1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the NRG1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-11-22
Question
Will EK1378 cross-react with mouse NRG1-beta 1?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
Our lab technicians think it would most likely cross reacts with mouse NRG1-beta 1.
Boster Scientific Support
Answered: 2020-02-03
Question
Do you have a recommended number or density of cells to prepare for EK1378 assay in Cell culure supernants and Cell lysates?
Verified customer
Asked: 2019-05-12
Answer
For the Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine™ (EK1378), the number of cells we recommened for cell culure supernants and cell lysates is 106 cells/mL.
Boster Scientific Support
Answered: 2019-05-12
Question
Q: how to thaw whole blood sample for NRG1 ELISA after freezing?
T. Collins
Verified customer
Asked: 2019-04-26
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test NRG1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-04-26
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
M. Nelson
Verified customer
Asked: 2019-01-09
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-01-09
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-10-17
Question
Q: What is the optimal O.D. value for NRG1 ELISA kit? I used your NRG1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain NRG1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-09-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this NRG1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of NRG1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-09-30
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-08-25
Question
Will EK1378 detects all neuregulin 1 isoforms or just the beta isoform?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
EK1378 detects just the beta isoform.
Boster Scientific Support
Answered: 2017-07-19
Question
Q: if the enzyme conjugated NRG1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the NRG1 antigen?
Verified Customer
Verified customer
Asked: 2016-04-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-04-19
Question
A customer is wondering if EK1378 detects all neuregulin 1 isoforms or just the beta isoform as per the title?
Verified Customer
Verified customer
Asked: 2013-06-18
Answer
EK1378 detects just the beta isoform.
Boster Scientific Support
Answered: 2013-06-18
