Anti-MUC1 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody

Product Name

Anti-MUC1 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody

View all MUC1 Antibodies

SKU/Catalog Number

M00187

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-MUC1 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody (Catalog # M00187). Tested in ELISA, Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-MUC1 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00187)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM493

Isotype

IgG1, kappa

Immunogen

Breast Cancer Cell line ZR75-1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00187 is reactive to MUC1 in Human, Mouse

Applications

M00187 is guaranteed for ELISA, Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

122102 MW

Background of MUC1

This monoclonal antibody reacts with MUC1. The dominant epitope of This monoclonal antibody has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (For coating use Ab at 2-5ug/ml, order Ab without BSA) (Very good capturing Ab)
Flow Cytometry (0.25-0.5ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Anti-MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (SPM493).

Gene/Protein Information For MUC1 (Source: Uniprot.org, NCBI)

Alternative Names

Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin MUC1 ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X/ZD, PEM, PEMT, PUM, MUC1 mucin 1, cell surface associated mucin-1|H23 antigen|breast carcinoma-associated antigen DF3|cancer antigen 15-3|carcinoma-associated mucin|episialin|krebs von den Lungen-6|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor associated epithelial mucin|tumor-associated epithelial membrane antigen

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