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Product Info Summary
| SKU: | EK2022 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Matrilin-3 ELISA Kit PicoKine®
SKU/Catalog Number
EK2022
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Matrilin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MATN3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Matrilin-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2022)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D29-R486
Sensitivity
<50 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2022 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2022 is reactive to MATN3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2022 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MATN3
Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 2p24.1. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2022-CAP | Anti-Human MATN3 Pre-coated 96-well strip microplate | 1 |
| EK2022-ST | Human MATN3 Standard | 2 vials, 10 ng/tube |
| EK2022-DA | Human MATN3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.110 | 0.183 | 0.230 | 0.386 | 0.596 | 0.991 | 1.661 | 2.295 |
Data Example Images
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Human Matrilin-3 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MATN3, Dilution ratio of 1:1, concentration in serum and plasma is around 250 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Matrilin-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 125 | 689 | 2209 | 148 | 697 | 2553 |
| Standard deviation | 6.13 | 29.63 | 90.57 | 9.77 | 40.43 | 194.03 |
| CV (%) | 4.9% | 4.3% | 4.1% | 6.6% | 5.8% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MATN3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 125 | 143 | 138 | 129 | 133 | 7.11 | 5.3% |
| Sample 2 | 689 | 800 | 745 | 802 | 759 | 46.43 | 6.1% |
| Sample 3 | 2209 | 2281 | 2219 | 2542 | 2312 | 135.20 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For MATN3 (Source: Uniprot.Org, NCBI)
Gene Name
MATN3
Full Name
Matrilin-3
Weight
Alternative Names
DIPOA; EDM5; HOA; matrilin 3; matrilin-3; OADIP; OS2 MATN3 DIPOA, EDM5, HOA, OADIP, OS2, SEMDBCD matrilin 3 matrilin-3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MATN3, check out the MATN3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MATN3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Matrilin-3 ELISA Kit PicoKine® (EK2022)
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Customer Q&As
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8 Customer Q&As for Human Matrilin-3 ELISA Kit PicoKine®
Question
Has EK2022 been validated for human samples?
Verified customer
Asked: 2019-09-05
Answer
The Human Matrilin-3 ELISA Kit PicoKine™ (EK2022) has been validated for human samples of the following: cell culture supernates, serum and plasma (heparin, EDTA).
Boster Scientific Support
Answered: 2019-09-05
Question
Q: What is the optimal O.D. value for MATN3 ELISA kit? I performed your MATN3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain MATN3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MATN3 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of MATN3 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-12-12
Question
Q: how can I thaw whole blood sample for MATN3 ELISA after freezing?
C. Thomas
Verified customer
Asked: 2018-11-22
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MATN3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-11-22
Question
I have an inquiry for Human Matrilin-3 ELISA Kit. (Cat # : EK2022) Is the kit validated? I would like to receive the validation data by e-mail.
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
COA is sent.
Boster Scientific Support
Answered: 2018-11-06
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-10-09
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-12-30
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-12-30
Question
Q: if the enzyme conjugated MATN3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MATN3 antigen?
Verified Customer
Verified customer
Asked: 2016-05-01
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-05-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2015-11-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-11-06
