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Product Info Summary
| SKU: | EK2024 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat Matrilin-3 ELISA Kit PicoKine®
SKU/Catalog Number
EK2024
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Matrilin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Matn3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Matrilin-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2024)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A28-R481
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2024 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2024 is reactive to Matn3 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2024 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MATN3
Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 6q14. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2024-CAP | Anti-Rat Matn3 Pre-coated 96-well strip microplate | 1 |
| EK2024-ST | Rat Matn3 Standard | 2 vials, 10 ng/tube |
| EK2024-DA | Rat Matn3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.110 | 0.183 | 0.230 | 0.386 | 0.596 | 0.991 | 1.661 | 2.095 |
Data Example Images
Click image to see more details
Rat Matrilin-3 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MATN3, Dilution ratio of 1:1, concentration in serum and plasma is around 400 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Matrilin-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 247 | 1165 | 4441 | 303 | 1139 | 4756 |
| Standard deviation | 12.10 | 50.10 | 182.08 | 18.18 | 66.06 | 328.16 |
| CV (%) | 4.9% | 4.3% | 4.1% | 6.0% | 5.8% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MATN3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 247 | 249 | 217 | 254 | 241 | 14.51 | 6.0% |
| Sample 2 | 1165 | 1151 | 1031 | 1075 | 1105 | 54.97 | 4.9% |
| Sample 3 | 4441 | 5053 | 4576 | 4552 | 4655 | 235.07 | 5.0% |
Protein Target Info & Infographic
Gene/Protein Information For Matn3 (Source: Uniprot.Org, NCBI)
Gene Name
Matn3
Full Name
Matrilin-3
Weight
52.817kDa
Alternative Names
Matrilin 3; Matn3 MATN3 DIPOA, EDM5, HOA, OADIP, OS2, SEMDBCD matrilin 3 matrilin-3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Matn3, check out the Matn3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Matn3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Matrilin-3 ELISA Kit PicoKine® (EK2024)
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Customer Q&As
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5 Customer Q&As for Rat Matrilin-3 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
K. Maharaj
Verified customer
Asked: 2020-09-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-09-24
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring MATN3 using the EK2024 Rat MATN3 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-08-08
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-08-08
Question
Q: can I use citrate plasma as samples in Rat MATN3 Picokine® ELISA Kit (Catalog # EK2024)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of MATN3 causing disruption of its protein structure. MATN3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the MATN3 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-12-16
Question
Q: What is the optimal O.D. value for MATN3 ELISA kit? I used your MATN3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain MATN3 even though the O.D. values are not very high?
S. Kulkarni
Verified customer
Asked: 2019-11-22
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MATN3 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of MATN3 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-11-22
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
G. Liu
Verified customer
Asked: 2016-01-25
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-01-25
