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Product Info Summary
| SKU: | PA1927 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Matrilin 3/MATN3 Antibody Picoband®
SKU/Catalog Number
PA1927
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Matrilin 3/MATN3 Antibody catalog # PA1927. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Matrilin 3/MATN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1927)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Matrilin 3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1927 is reactive to MATN3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
71 kDa
Calculated molecular weight
52817 MW
Background of MATN3
MATN3 (Matrilin-3) is a protein that in humans is encoded by the MATN3 gene. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is though to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. By fluorescence in situ hybridization, Belluoccio et al. (1998) mapped the human MATN3 gene to chromosome 2p24-p23. Wagener et al. (2000) mapped the mouse Matn3 gene to the proximal end of chromosome 12, linked to the genes Synd1, Apob, Dtnb, and Kif3c. The human homologs of all 5 of these genes map to 2p23, indicating considerable homology of synteny. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1927 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: 293T Cell, COLO320 Cell, HELA Cell, A549 Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Matrilin 3 antibody, PA1927, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For MATN3 (Source: Uniprot.org, NCBI)
Gene Name
MATN3
Full Name
Matrilin-3
Weight
52817 MW
Alternative Names
Matrilin-3;MATN3; MATN3 DIPOA, EDM5, HOA, OADIP, OS2, SEMDBCD matrilin 3 matrilin-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MATN3, check out the MATN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MATN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Matrilin 3/MATN3 Antibody Picoband® (PA1927)
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No publications found for PA1927
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Customer Q&As
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6 Customer Q&As for Anti-Matrilin 3/MATN3 Antibody Picoband®
Question
Do you have a BSA free version of anti-Matrilin 3/MATN3 antibody PA1927 available?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Matrilin 3/MATN3 antibody PA1927 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-23
Question
I was wanting to use your anti-Matrilin 3/MATN3 antibody for WB for human tibia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tibia identification?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
You can see on the product datasheet, PA1927 anti-Matrilin 3/MATN3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tibia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-11
Question
Does anti-Matrilin 3/MATN3 antibody PA1927 work on bovine WB with cartilage?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Our lab technicians have not validated anti-Matrilin 3/MATN3 antibody PA1927 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Matrilin 3/MATN3 antibody PA1927 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cartilage in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
My lab would like to test anti-Matrilin 3/MATN3 antibody PA1927 on human tibia for research purposes, then I may be interested in using anti-Matrilin 3/MATN3 antibody PA1927 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
The products we sell, including anti-Matrilin 3/MATN3 antibody PA1927, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-16
Question
My question regarding product PA1927, anti-Matrilin 3/MATN3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1927 anti-Matrilin 3/MATN3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-17
Question
We are currently using anti-Matrilin 3/MATN3 antibody PA1927 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
B. Wu
Verified customer
Asked: 2016-11-17
Answer
The anti-Matrilin 3/MATN3 antibody (PA1927) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-11-17
