Product Info Summary
SKU: | EK2237 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human LRIG1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2237
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LRIG1 PicoKine ELISA Kit® (96 Tests). Quantitate Human LRIG1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LRIG1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2237)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: HEK293; Immunogen sequence: A35-S779
Sensitivity
<15 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2237 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2237 is reactive to Lrig1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2237 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lrig1
Leucine-rich repeats and immunoglobulin-like domains protein 1 is a protein that in humans is encoded by the LRIG1 gene. It encodes atransmembrane proteinthat has been shown to interact with receptor tyrosine kinases of theEGFR-family,METandRET. Acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK2237-CAP | Anti-Human Lrig1 Pre-coated 96-well strip microplate | 1 |
EK2237-ST | Human Lrig1 Standard | 2 vials, 10 ng/tube |
EK2237-DA | Human Lrig1 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
O.D. | 0.025 | 0.064 | 0.106 | 0.315 | 0.721 | 1.353 | 1.516 | 2.043 |
Data Example Images
![ek2237 ek2237](https://www.bosterbio.com/media/catalog/product/e/k/ek2237.png)
Click image to see more details
Human LRIG1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LRIG1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 44 | 70 | 885 | 47 | 83 | 924 |
Standard deviation | 3.14 | 2.6 | 34.69 | 2.35 | 3.49 | 41.67 |
CV(%) | 7.1% | 3.7% | 3.9% | 5.0% | 4.2% | 4.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Lrig1 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 44 | 37 | 42 | 45 | 42 | 1.76 | 4.2% |
Sample 2 | 70 | 73 | 124 | 117 | 96 | 4.32 | 4.5% |
Sample 3 | 885 | 953 | 982 | 700 | 880 | 25.17 | 2.9% |
Protein Target Info & Infographic
Gene/Protein Information For Lrig1 (Source: Uniprot.Org, NCBI)
Gene Name
Lrig1
Full Name
Leucine-rich repeats and immunoglobulin-like domains protein 1
Weight
Alternative Names
D6Bwg0781e; Img; LIG1; LIG-1; LRIG1 Lrig1|D6Bwg0781e, Im, Img, LIG, LIG-1|leucine-rich repeats and immunoglobulin-like domains 1|leucine-rich repeats and immunoglobulin-like domains protein 1|integral membrane glycoprotein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Lrig1, check out the Lrig1 Infographic
![Lrig1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Lrig1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human LRIG1 ELISA Kit PicoKine® (EK2237)
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