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Product Info Summary
| SKU: | A00490-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-syk (Ab-323) Antibody
SKU/Catalog Number
A00490-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-syk (Ab-323) Antibody (Catalog # A00490-2). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-syk (Ab-323) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00490-2)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 321~325 (N-P-Y-E-P) derived from Human syk.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00490-2 is reactive to SYK in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
71245 MW
Background of SYK
Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion
Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.
Turner, M. et al. (2000) Immunol. Today 21, 148-154.
Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00490-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 72kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SYK using anti-SYK antibody (A00490-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYK antigen affinity purified polyclonal antibody (Catalog # A00490-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SYK.
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Immunofluorescence staining of methanol-fixed Hela cells using syk(Ab-323) Antibody #A00490-2.
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Figure 2. IHC analysis of SYK using anti-SYK antibody (A00490-2).
SYK was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SYK Antibody (A00490-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SYK (Source: Uniprot.org, NCBI)
Gene Name
SYK
Full Name
Tyrosine-protein kinase SYK
Weight
71245 MW
Superfamily
protein kinase superfamily
Alternative Names
Thrombopoietin receptor;TPO-R;Myeloproliferative leukemia protein;Proto-oncogene c-Mpl;CD110;MPL;TPOR; SYK p72-Syk spleen associated tyrosine kinase tyrosine-protein kinase SYK|spleen tyrosine kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYK, check out the SYK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-syk (Ab-323) Antibody (A00490-2)
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Customer Q&As
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16 Customer Q&As for Anti-syk (Ab-323) Antibody
Question
Would A00490-2 anti-syk (Ab-323) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
You can see on the product datasheet, A00490-2 anti-syk (Ab-323) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-13
Question
I would like to test anti-syk (Ab-323) antibody A00490-2 on human b-cell for research purposes, then I may be interested in using anti-syk (Ab-323) antibody A00490-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The products we sell, including anti-syk (Ab-323) antibody A00490-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-30
Question
My question regards using your anti-syk (Ab-323) antibody for positive regulation of cell adhesion mediated by integrin studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
I appreciate your inquiry. This A00490-2 anti-syk (Ab-323) antibody is validated on hela cells. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-04
Question
I see that the anti-syk (Ab-323) antibody A00490-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-23
Question
Is there a BSA free version of anti-syk (Ab-323) antibody A00490-2 available?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-syk (Ab-323) antibody A00490-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-17
Question
My question regarding product A00490-2, anti-syk (Ab-323) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Parker
Verified customer
Asked: 2019-11-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00490-2 anti-syk (Ab-323) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-01
Question
I was wanting to use your anti-syk (Ab-323) antibody for WB for human b-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human b-cell identification?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
You can see on the product datasheet, A00490-2 anti-syk (Ab-323) antibody has been tested for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-27
Question
We purchased anti-syk (Ab-323) antibody for WB on tonsil in a previous experiment. I am using human, and We intend to use the antibody for IHC next. My lab would like examining tonsil as well as eye lymph in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
I viewed the website and datasheets of our anti-syk (Ab-323) antibody and I see that A00490-2 has been tested on human in both WB and IHC. Thus A00490-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-05
Question
We have been able to see staining in human b-cell. Are there any suggestions? Is anti-syk (Ab-323) antibody supposed to stain b-cell positively?
H. Yang
Verified customer
Asked: 2019-01-22
Answer
Based on literature b-cell does express SYK. Based on Uniprot.org, SYK is expressed in leukocyte, eye lymph, tonsil, platelet, leukemic t-cell, b-cell, among other tissues. Regarding which tissues have SYK expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 21469132
Eye, and Lymph, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Platelet, Pubmed ID: 18088087
Tonsil, Pubmed ID: 8168854
Boster Scientific Support
Answered: 2019-01-22
Question
Our lab were satisfied with the WB result of your anti-syk (Ab-323) antibody. However we have seen positive staining in leukocyte cell membrane. cytoplasm, using this antibody. Is that expected? Could you tell me where is SYK supposed to be expressed?
J. Krishna
Verified customer
Asked: 2018-07-27
Answer
According to literature, leukocyte does express SYK. Generally SYK expresses in cell membrane. cytoplasm,. Regarding which tissues have SYK expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 21469132
Eye, and Lymph, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Platelet, Pubmed ID: 18088087
Tonsil, Pubmed ID: 8168854
Boster Scientific Support
Answered: 2018-07-27
Question
Will anti-syk (Ab-323) antibody A00490-2 work for WB with b-cell?
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
According to the expression profile of b-cell, SYK is highly expressed in b-cell. So, it is likely that anti-syk (Ab-323) antibody A00490-2 will work for WB with b-cell.
Boster Scientific Support
Answered: 2018-05-14
Question
Is this A00490-2 anti-syk (Ab-323) antibody reactive to the isotypes of SYK?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
The immunogen of A00490-2 anti-syk (Ab-323) antibody is Peptide sequence around aa. 321~325 (N-P-Y-E-P) derived from Human syk. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-15
Question
See below the WB image, lot number and protocol we used for b-cell using anti-syk (Ab-323) antibody A00490-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-25
Question
Is a blocking peptide available for product anti-syk (Ab-323) antibody (A00490-2)?
B. Anderson
Verified customer
Asked: 2016-08-23
Answer
We do provide the blocking peptide for product anti-syk (Ab-323) antibody (A00490-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-08-23
Question
We are currently using anti-syk (Ab-323) antibody A00490-2 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
C. Li
Verified customer
Asked: 2013-08-13
Answer
The anti-syk (Ab-323) antibody (A00490-2) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-13
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-syk (Ab-323) antibody A00490-2. Let me know if you need anything else.
E. Bhatt
Verified customer
Asked: 2013-03-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-03-06
