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Product Info Summary
| SKU: | A00490Y525-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Phospho-Syk (Y525) Antibody
SKU/Catalog Number
A00490Y525-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Syk (Y525) Antibody catalog # A00490Y525-1. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Syk (Y525) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00490Y525-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SYK around the phosphorylation site of Tyr525. AA range:491-540
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00490Y525-1 is reactive to SYK in Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
72066 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00490Y525-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-Syk (Y525) polyclonal antibody
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SYK (Phospho-Tyr525) antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SYK (Phospho-Tyr525) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from A549 cells, using SYK (Phospho-Tyr525) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For SYK (Source: Uniprot.org, NCBI)
Gene Name
SYK
Full Name
Tyrosine-protein kinase SYK
Weight
72066 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase SYK;2.7.10.2;Spleen tyrosine kinase;p72-Syk;SYK; SYK p72-Syk spleen associated tyrosine kinase tyrosine-protein kinase SYK|spleen tyrosine kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYK, check out the SYK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Syk (Y525) Antibody (A00490Y525-1)
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