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Product Info Summary
| SKU: | EK0416 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
Product info
Product Name
Human IL-10/Interleukin-10 ELISA Kit PicoKine®
SKU/Catalog Number
EK0416
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-10/Interleukin-10 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL10 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-10/Interleukin-10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0416)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S19-N178
Sensitivity
<0.5 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of EK0416 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0416 is reactive to IL10 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
Application Guarantee
EK0416 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-10
Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0416-CAP | Anti-Human IL10 Pre-coated 96-well strip microplate | 1 |
| EK0416-ST | Human IL10 Standard | 2 vials, 10 ng/tube |
| EK0416-DA | Human IL10 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.060 | 0.101 | 0.151 | 0.187 | 0.295 | 0.538 | 0.858 | 1.722 |
Data Example Images
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Human IL-10 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-10, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 7.8 pg/ml..
Some articles we found to cite concentrations of IL-10 in samples: 21878171 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-10/Interleukin-10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 16 | 53 | 113 | 15 | 54 | 122 |
| Standard deviation | 1.02 | 2.12 | 7.34 | 1.24 | 3.02 | 9.88 |
| CV (%) | 6.4% | 4% | 6.5% | 8.3% | 5.6% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-10 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 16 | 17 | 15 | 15 | 15 | 0.82 | 5.4% |
| Sample 2 | 53 | 45 | 47 | 48 | 48 | 2.94 | 6.1% |
| Sample 3 | 113 | 123 | 106 | 104 | 111 | 7.43 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For IL10 (Source: Uniprot.Org, NCBI)
Gene Name
IL10
Full Name
Interleukin-10
Weight
20517 MW
Superfamily
IL-10 family
Alternative Names
Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;IL10; IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL10, check out the IL10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-10/Interleukin-10 ELISA Kit PicoKine® (EK0416)
Hello CJ!
EK0416 has been cited in 160 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155
Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery
Therapeutic effects of ciprofloxacin/bushenhuazhuo combination on chronic prostatitis
Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome
Bladder cancer cell‑secreted exosomal miR‑21 activates the PI3K/AKT pathway in macrophages to promote cancer progression
The effect of intravascular interventional embolization and craniotomy on MMP-2, MMP-9 and caspase3 in serum of intracranial aneurysm patients
Morphological and functional changes in bone marrow mesenchymal stem cells in rats with heart failure
Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome
Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations
FoxP3 - Tr1 Cell in Generalized Myasthenia Gravis and Its Relationship With the Anti-AChR Antibody and Immunomodulatory Cytokines
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
12 Customer Q&As for Human IL-10/Interleukin-10 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated IL-10 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-10 antigen?
Verified Customer
Verified customer
Asked: 2020-12-08
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-12-08
Question
Q: can you recommend the dilution ratio of serum samples for detection of IL-10 in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Y. Li
Verified customer
Asked: 2020-03-09
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-10 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-09
Question
Q: Can IL-10 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Puri
Verified customer
Asked: 2019-07-23
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-07-23
Question
Have you tested EK0410 and EK0416 in tissue homogenates and Cell culture supernates?
Verified customer
Asked: 2019-03-15
Answer
The Human IL-6 EK0410 Elisa Kit and Human IL-10 EK0416 Elisa Kit have not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-03-18
Question
Q: is it okay to use EDTA plasma as samples in Human IL-10 Picokine® ELISA Kit (Catalog # EK0416)?
H. Smith
Verified customer
Asked: 2018-10-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-10 causing degradation of its protein structure. IL-10 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IL-10 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-01
Question
My sample type is cell supernates. I would like to know what ratio should I use to dilute my samples during using your product EK0416 and EK0525. I couldn't find any reference from the product page.
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
For untreated cell supernatants, the suggested dilution ratio is 1:1. For treated cell supernatants, concentrations of the samples can vary greatly. We suggest the customer run pilot tests for different group samples to figure out the optimal dilution ratio.
Boster Scientific Support
Answered: 2018-08-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-08-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-08-05
Question
I am looking for IL 10 that works both with human and mouse is the EK0416 antibody kit will cross react with human and mouse?
Verified Customer
Verified customer
Asked: 2018-06-21
Answer
The EK0416 antibody kit did not show any cross-reactivity with mouse samples, therefore, this kit is not expected to cross react.
Boster Scientific Support
Answered: 2018-06-21
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-06-16
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-06-16
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-08
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
M. Walker
Verified customer
Asked: 2017-11-25
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-11-25
Question
Q: how can I thaw whole blood sample for IL-10 ELISA after freezing?
N. Moore
Verified customer
Asked: 2017-10-22
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-10 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-10-22
