Anti-CD50 ICAM3 Antibody

Product Name

Anti-CD50 ICAM3 Antibody

View all ICAM-3/CD50 Antibodies

SKU/Catalog Number

A03651

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CD50 ICAM3 Antibody catalog # A03651. Tested in ELISA, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD50 ICAM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03651)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human CD50 around the non-phosphorylation site of S518.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03651 is reactive to ICAM3 in Human

Applications

A03651 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

59541 MW

Background of ICAM-3/CD50

Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.

Hongzhe Li, J. Biol. Chem., Feb 2007; 282: 4943 - 4950.
Wuhan Xiao, Cancer Res., Aug 2003; 63: 4698 - 4704.
Thierry Virolle, J. Biol. Chem., Mar 2003; 278: 11802 - 11810.
Derek J. Richard, Genes & Dev., Feb 2001; 15: 328.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-CD50 ICAM3 Antibody A03651

Western Blot (WB) analysis of NIH-3T3 cells using CD50 polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For ICAM3 (Source: Uniprot.org, NCBI)

Alternative Names

CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3 ICAM3 CD50, CDW50, ICAM-R intercellular adhesion molecule 3 intercellular adhesion molecule 3|ICAM-3

For more info on ICAM3, check out the ICAM3 Infographic

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In this infographic, you will see the following information for ICAM3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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