Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®

Product Name

Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®

View all Iduronate 2-Sulfatase/IDS ELISA kits

SKU/Catalog Number

EK1452

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® (96 Tests). Quantitate Human IDS in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1452)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: S26-P550

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

See datasheet of EK1452 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1452 is reactive to IDS in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1452 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Iduronate 2-Sulfatase/IDS

Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Kit Components

Catalog Number	Description	Quantity
EK1452-CAP	Anti-Human IDS Pre-coated 96-well strip microplate	1
EK1452-ST	Human IDS Standard	2 vials, 10 ng/tube
EK1452-DA	Human IDS Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.030	0.071	0.165	0.291	0.510	0.802	1.350	1.921

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Iduronate 2-Sulfatase/IDS, Dilution ratio of 1:10, concentration in serum and plasma is 13.7-38.3 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Iduronate 2-Sulfatase/IDS in ELISA kits from four different production batches/lots.

Gene/Protein Information For IDS (Source: Uniprot.Org, NCBI)

Alternative Names

Iduronate 2-sulfatase;3.1.6.13;Alpha-L-iduronate sulfate sulfatase;Idursulfase;Iduronate 2-sulfatase 42 kDa chain;Iduronate 2-sulfatase 14 kDa chain;IDS;SIDS; IDS ID2S, MPS2, SIDS iduronate 2-sulfatase iduronate 2-sulfatase|alpha-L-iduronate sulfate sulfatase|iduronate 2-sulfatase 14 kDa chain|iduronate 2-sulfatase 42 kDa chain|idursulfase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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