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Product Info Summary
| SKU: | PA1917 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Iduronate 2 sulfatase/IDS Antibody Picoband®
View all Iduronate 2-Sulfatase/IDS Antibodies
SKU/Catalog Number
PA1917
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Iduronate 2 sulfatase/IDS Antibody catalog # PA1917. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Iduronate 2 sulfatase/IDS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1917)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Iduronate 2 sulfatase.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1917 is reactive to IDS in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22-24 kDa
Calculated molecular weight
61873 MW
Background of Iduronate 2-Sulfatase/IDS
IDS (Iduronate-2-sulfatase) is a sulfatase enzyme associated with Hunter syndrome. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. Wilson et al. (1991) used an IDS cDNA clone to localize the gene to Xq28, distal to the fragile X site. Faust et al. (1992) and Daniele et al. (1993) demonstrated that the homologous Ids gene in the mouse occupies the same position on the X chromosome in relation to the FMR1, F9, and GABRA3 genes. Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked mucopolysaccharidosis type II, also known as Hunter syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1917 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Cell, SMMC Cell, A549 Cell, MCF-7 Cell, COLO Cell
IHC: Human Placenta Tissue
Validation Images & Assay Conditions
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Anti-Iduronate 2 sulfatase antibody, PA1917, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: COLO Cell Lysate
Click image to see more details
Anti-Iduronate 2 sulfatase antibody, PA1917, IHC(P)
IHC(P): Human Placenta Tissue
Protein Target Info & Infographic
Gene/Protein Information For IDS (Source: Uniprot.org, NCBI)
Gene Name
IDS
Full Name
Iduronate 2-sulfatase
Weight
61873 MW
Superfamily
sulfatase family
Alternative Names
Iduronate 2-sulfatase;3.1.6.13;Alpha-L-iduronate sulfate sulfatase;Idursulfase;Iduronate 2-sulfatase 42 kDa chain;Iduronate 2-sulfatase 14 kDa chain;IDS;SIDS; IDS ID2S, MPS2, SIDS iduronate 2-sulfatase iduronate 2-sulfatase|alpha-L-iduronate sulfate sulfatase|iduronate 2-sulfatase 14 kDa chain|iduronate 2-sulfatase 42 kDa chain|idursulfase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IDS, check out the IDS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IDS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Iduronate 2 sulfatase/IDS Antibody Picoband® (PA1917)
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No publications found for PA1917
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Iduronate 2 sulfatase/IDS Antibody Picoband®
Question
Does PA1917 anti-Iduronate 2 sulfatase/IDS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can see on the product datasheet, PA1917 anti-Iduronate 2 sulfatase/IDS antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-07
Question
Is this PA1917 anti-Iduronate 2 sulfatase/IDS antibody reactive to the isotypes of IDS?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The immunogen of PA1917 anti-Iduronate 2 sulfatase/IDS antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Iduronate 2 sulfatase(430-448aa ELCREGKNLLKHFRFRDLE). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-16
Question
Is a blocking peptide available for product anti-Iduronate 2 sulfatase/IDS antibody (PA1917)?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We do provide the blocking peptide for product anti-Iduronate 2 sulfatase/IDS antibody (PA1917). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Iduronate 2 sulfatase/IDS antibody PA1917. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-14
Question
I am interested in to test anti-Iduronate 2 sulfatase/IDS antibody PA1917 on human liver for research purposes, then I may be interested in using anti-Iduronate 2 sulfatase/IDS antibody PA1917 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The products we sell, including anti-Iduronate 2 sulfatase/IDS antibody PA1917, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-15
Question
I was wanting to use your anti-Iduronate 2 sulfatase/IDS antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
It shows on the product datasheet, PA1917 anti-Iduronate 2 sulfatase/IDS antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-02
Question
Can you help my question with product PA1917, anti-Iduronate 2 sulfatase/IDS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1917 anti-Iduronate 2 sulfatase/IDS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-01
Question
Would anti-Iduronate 2 sulfatase/IDS antibody PA1917 work on canine WB with lymphocyte?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
Our lab technicians have not tested anti-Iduronate 2 sulfatase/IDS antibody PA1917 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Iduronate 2 sulfatase/IDS antibody PA1917 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine lymphocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-24
Question
Please see the WB image, lot number and protocol we used for liver using anti-Iduronate 2 sulfatase/IDS antibody PA1917. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-30
Question
We purchased anti-Iduronate 2 sulfatase/IDS antibody for WB on skin in the past. I am using human, and We are going to use the antibody for IHC next. you antibody examining skin as well as lymphocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-04-02
Answer
I looked at the website and datasheets of our anti-Iduronate 2 sulfatase/IDS antibody and it seems that PA1917 has been tested on human in both WB and IHC. Thus PA1917 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-04-02
Question
We were satisfied with the WB result of your anti-Iduronate 2 sulfatase/IDS antibody. However we have been able to see positive staining in skin lysosome. using this antibody. Is that expected? Could you tell me where is IDS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
According to literature, skin does express IDS. Generally IDS expresses in lysosome. Regarding which tissues have IDS expression, here are a few articles citing expression in various tissues:
Endothelial cell, Pubmed ID: 2122463
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 8530090
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-04-09
Question
I see that the anti-Iduronate 2 sulfatase/IDS antibody PA1917 works with WB, what is the protocol used to produce the result images on the product page?
W. Krishna
Verified customer
Asked: 2018-03-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-19
Question
We have seen staining in human skin. Any tips? Is anti-Iduronate 2 sulfatase/IDS antibody supposed to stain skin positively?
K. Gonzalez
Verified customer
Asked: 2017-10-12
Answer
From literature skin does express IDS. From Uniprot.org, IDS is expressed in endothelial cell, lymphocyte, skin, liver, among other tissues. Regarding which tissues have IDS expression, here are a few articles citing expression in various tissues:
Endothelial cell, Pubmed ID: 2122463
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 8530090
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-10-12
Question
Does anti-Iduronate 2 sulfatase/IDS antibody PA1917 work for WB with liver?
F. Singh
Verified customer
Asked: 2015-09-07
Answer
According to the expression profile of liver, IDS is highly expressed in liver. So, it is likely that anti-Iduronate 2 sulfatase/IDS antibody PA1917 will work for WB with liver.
Boster Scientific Support
Answered: 2015-09-07
Question
Is there a BSA free version of anti-Iduronate 2 sulfatase/IDS antibody PA1917 available?
C. Krishna
Verified customer
Asked: 2014-06-05
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Iduronate 2 sulfatase/IDS antibody PA1917 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-06-05
Question
We are currently using anti-Iduronate 2 sulfatase/IDS antibody PA1917 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
O. Carter
Verified customer
Asked: 2014-01-07
Answer
The anti-Iduronate 2 sulfatase/IDS antibody (PA1917) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-01-07
Question
We are interested in using your anti-Iduronate 2 sulfatase/IDS antibody for hs-gag degradation studies. Has this antibody been tested with western blotting on a549 cell lysate? We would like to see some validation images before ordering.
K. Li
Verified customer
Asked: 2013-02-22
Answer
We appreciate your inquiry. This PA1917 anti-Iduronate 2 sulfatase/IDS antibody is tested on hela cell lysate, smmc cell lysate, a549 cell lysate, human placenta tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-02-22
