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Key=assay_range, value=156 pg/ml - 10,000 pg/ml
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Key=principle, value=monoclonal antibody from mouse|polyclonal antibody from goat
Key=anticoagulant, value=heparin or EDTA
Key=assay_procedure_step_1, value=10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml
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Key=description, value=Human Galectin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Human Galectin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=meta_keyword, value=Human Galectin-2 ELISA Kit PicoKine, Bosterbio High Sensitivity
Key=background, value=Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.
Key=gene_name, value=LGALS2
Key=synonyms, value=Galectin-2;Gal-2;Beta-galactoside-binding lectin L-14-II;HL14;Lactose-binding lectin 2;S-Lac lectin 2;LGALS2;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=10 ng/tube
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  • Microplate reader in standard size.
  • Automated plate washer.
  • Incubator.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

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Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2881975512826318485204
Standard deviation21.31100.72302.5523.4129.36369.48
CV (%)7.4%7.1%5.9%8.9%7%7.1%

Key=application_details, value=Dilution ratio of 1:1, concentration in serum and plasma is 740-1226 pg/ml.
Key=sequence_similarities, value=Contains 1 galectin domain.
Key=protein_function, value= This protein binds beta-galactoside. Its physiological function is not yet known.
Key=sample_data, value=
Concentration (pg/ml)0156312625125025005,00010,000
O.D.0.0280.0980.1430.2580.5260.9361.5252.002

Key=competitor_equivalent_skus, value=
Key=immunogen, value=Expression system for standard: E.coli; Immunogen sequence: M1-E132
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128832333132631716.985.3%
Sample 21975197418981989195935.711.8%
Sample 351285263528245545056296.275.8%
*number of samples for each test n=16.
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Human Galectin-2 ELISA Kit PicoKine®

Galectin-2 ELISA kit for Human

Human Galectin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK1278
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Galectin-2 ELISA Kit PicoKine®

View all Galectin-2 ELISA kits

SKU/Catalog Number

EK1278

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Galectin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Galectin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1278)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: M1-E132

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK1278 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1278 is reactive to LGALS2 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1278 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Galectin-2

Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.

Kit Components

Catalog Number Description Quantity
EK1278-CAP Anti-Human LGALS2 Pre-coated 96-well strip microplate 1
EK1278-ST Human LGALS2 Standard 2 vials, 10 ng/tube
EK1278-DA Human LGALS2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0156312625125025005,00010,000
O.D.0.0280.0980.1430.2580.5260.9361.5252.002

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Galectin-2, Dilution ratio of 1:1, concentration in serum and plasma is 740-1226 pg/ml..

Some articles we found to cite concentrations of Galectin-2 in samples: 21933892 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Galectin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2881975512826318485204
Standard deviation21.31100.72302.5523.4129.36369.48
CV (%)7.4%7.1%5.9%8.9%7%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Galectin-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128832333132631716.985.3%
Sample 21975197418981989195935.711.8%
Sample 351285263528245545056296.275.8%
*number of samples for each test n=16.

Gene/Protein Information For LGALS2 (Source: Uniprot.Org, NCBI)

Gene Name

LGALS2

Full Name

Galectin-2

Weight

14644 MW

Alternative Names

Galectin-2;Gal-2;Beta-galactoside-binding lectin L-14-II;HL14;Lactose-binding lectin 2;S-Lac lectin 2;LGALS2; LGALS2 HL14 galectin 2 galectin-2|S-Lac lectin 2|beta-galactoside-binding lectin L-14-II|gal-2|lactose-binding lectin 2|lectin, galactoside-binding, soluble, 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LGALS2, check out the LGALS2 Infographic

LGALS2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LGALS2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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10 Customer Q&As for Human Galectin-2 ELISA Kit PicoKine®

Question

Does EK1278 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Human Galectin-2 ELISA Kit PicoKine® (EK1278) doesn't contain native human biological materials. doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

C. Yang

Verified customer

Asked: 2020-12-23

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-12-23

Question

Q: Can Galectin-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-12-18

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-12-18

Question

Q: can you recommend the dilution ratio of serum samples for detection of Galectin-2 in Human cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

L. Dayal

Verified customer

Asked: 2020-01-09

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Galectin-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-01-09

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-03-01

Question

Q: how can I thaw whole blood sample for Galectin-2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-04-14

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Galectin-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-04-14

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-08-28

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-08-28

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

J. Campbell

Verified customer

Asked: 2015-08-24

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-08-24

Question

Q: What is the optimal O.D. value for Galectin-2 ELISA kit? I used your Galectin-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Galectin-2 even though the O.D. values are not very high?

C. Bhatt

Verified customer

Asked: 2015-02-06

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Galectin-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Galectin-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2015-02-06

Question

Q: how to analyze ELISA data? I have obtained Galectin-2 level in cell lysates.

M. Young

Verified customer

Asked: 2014-06-20

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2014-06-20

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