Anti-FOLR2 Purified Monoclonal Antibody

Product Name

Anti-FOLR2 Purified Monoclonal Antibody

View all FOLR2 Antibodies

SKU/Catalog Number

M04574

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FOLR2 Purified Monoclonal Antibody (Catalog# M04574). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-FOLR2 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04574)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

EM-35

Isotype

Mouse IgG1

Immunogen

BW5147alpha,beta- cells. The mouse monoclonal antibody EM-35 recognizes an extracellular epitope on FOLR2, a 30-40 kDa cell surface protein serving as a receptor for folic acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M04574 is reactive to FOLR2 in Human

Applications

M04574 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

29280 MW

Background of FOLR2

FOLR2 (folate receptor beta) is a cell surface protein that was originally thought to be specific for placenta, but it can be also expressed in other tissues, including hematopoietic cells. Its expression is increased in malignant tissues. FOLR2 may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. FOLR2 is a marker for macrophages generated in the presence of M-CSF (M2), including M2-like tumor-associated macrophages, which exert potent immunosuppressive functions within the tumor environment, but not GM-CSF (M1), and whose expression correlates with increased folate uptake ability.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: Non-reducing conditions.
Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-FOLR2 Purified Monoclonal Antibody M04574

Western blotting analysis of FOLR2 in THP1 cells (A) and FOLR2-transfected THP1 cells (B) using anti-FOLR2 (EM-35) purified.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For FOLR2 (Source: Uniprot.org, NCBI)

Alternative Names

beta-HFR; FBP; FBP/PL-1; folate receptor 2 (fetal); Folate receptor 2; folate receptor beta; folate receptor, beta; Folate receptor, fetal/placental; folate-binding protein, fetal/placental; FOLR2; FR-beta; FR-P3; Placental folate-binding protein FOLR2 BETA-HFR, FBP, FBP/PL-1, FOLR1, FR-BETA, FR-P3, FRbeta folate receptor beta folate receptor beta|folate receptor 2 (fetal)|folate receptor alpha|folate receptor, fetal/placental|folate-binding protein, fetal/placental|placental folate-binding protein

For more info on FOLR2, check out the FOLR2 Infographic

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In this infographic, you will see the following information for FOLR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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