Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit

Dkk-1 ELISA kit, 90-minute one-step protocol

Human DKK-1/Dickkopf 1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human DKK-1/Dickkopf 1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: FEK0867
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Assay Time: 90 Minutes

How Quick Picokine(R) ELISA kits work: Get data in 90 minutes

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Product Name

Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit

See all Dkk-1 products

SKU/Catalog Number

FEK0867

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human DKK-1/Dickkopf 1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human DKK-1/Dickkopf 1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0867)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T32-H266

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0867 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0867 is reactive to Dkk1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0867 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Dkk-1

Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Kit Components

Catalog numberDescription QuantityVolume
FEK0867-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK0867-STHuman Dkk-1 Standard 2 10 ng/tube
FEK0867-AHuman Dkk1 Antibody A 1 3ml
FEK0867-BHuman Dkk1 Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)062.51252505001,0002,0004,000
O.D.0.0330.0620.1710.2530.4250.7331.2911.902

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)16665215181475641364
Standard deviation9.7929.34103.2248.6725.3892.75
CV(%)5.9%4.5%6.8%5.8%4.5%6.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Dkk-1 in ELISA kits from four different production batches/lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11661551671521603.522.2%
Sample 265269463767166431.854.8%
Sample 31518150312461377141163.54.5%
*number of samples for each test n=16.

Gene/Protein Information For Dkk1 (Source: Uniprot.Org, NCBI)

Gene Name

Dkk1

Full Name

Dickkopf-related protein 1

Weight

Superfamily

dickkopf family

Alternative Names

dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like Dkk1|mdkk-1|dickkopf WNT signaling pathway inhibitor 1|dickkopf-related protein 1|dickkopf homolog 1|dickkopf-1|dickkopf-like protein 1|dkk-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Dkk1, check out the Dkk1 Infographic

Dkk1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Dkk1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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