Human Cystatin B Recombinant Protein

Cystatin B/Stefin B protein,

Human Cystatin B Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-98aa. Application(s): Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Product Info Summary

SKU: PROTP04080
Size: Starting from 10ug
Source: E. coli
Application: Enzyme Activity, SDS-PAGE

Product Name

Human Cystatin B Recombinant Protein

View all Cystatin B/Stefin B recombinant proteins

SKU/Catalog Number

PROTP04080

Size

Starting from 10ug

Tag

His-Tag

Description

Human Cystatin B Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-98aa. Application(s): Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Storage & Handling

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Cite This Product

Human Cystatin B Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP04080)

Form

Liquid

Formulation

20mM Tris-HCl buffer (pH 8.0) containing 50mM NaCl

Concentration

1mg/ml (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Predicted MW

13 kDa (118aa) confirmed by MALDI-TOF

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Amino Acid Sequence

Human, P04080, 1-98aa; MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF

Bioactivity

The IC50 value is < 3.0nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.

Validation Images & Assay Conditions

Gene/Protein Information For CSTB (Source: Uniprot.org, NCBI)

Gene Name

CSTB

Full Name

Cystatin-B

Weight

13 kDa (118aa) confirmed by MALDI-TOF

Superfamily

cystatin family

Alternative Names

Cystatin-B, Stefin B, PME, CST6 CSTB CPI-B, CST6, EPM1, EPM1A, PME, STFB, ULD cystatin B cystatin-B|cystatin B (stefin B)|epididymis secretory sperm binding protein|liver thiol proteinase inhibitor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on CSTB, check out the CSTB Infographic

CSTB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CSTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $265

SKU:PROTP04080

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