Cystatin-B Antibodies

Cystatin-B (CSTB)

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

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Gene Information

Human
Gene Name:	CSTB
Uniprot:	P04080
Entrez:	1476

Family

Belongs to:
cystatin family

Alternative Names

CPI-B; CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B; CSTB; cystatin B (stefin B); Cystatin B; EPM1; Liver thiol proteinase inhibitor; PME; Stefin B; stefin-B

Molecular Weight

Mass (kDA):
11.14 kDA

Genomic Context

Human
Location:	21q22.3
Sequence:	21; NC_000021.9 (43773950..43776308, complement)

Subcellular Localizations

Cytoplasm. Nucleus.

Diseases

• Epilepsies, Myoclonic
• Epilepsy
• Myoclonic Epilepsies, Progressive
• Myoclonus
• Unverricht-lundborg Syndrome
• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Ataxia
• Neurodegenerative Disorders
• Malignant Paraganglionic Neoplasm
• Malignant Squamous Cell Neoplasm

• Tonic-clonic Epilepsy
• Neoplasm Metastasis
• Adenocarcinoma
• Head And Neck Carcinoma
• Head And Neck Neoplasms
• Atrophy
• Cell Invasion

Pathways

• Localization
• Pathogenesis
• Amyloid Fibril Formation
• Cell Cycle
• Proteolysis
• Cell Proliferation
• Immune Response
• Cell Death
• Translation
• Cell Differentiation
• Methylation
• Protein Folding
• S Phase

• Cell Growth
• Bone Resorption
• Autophagy
• Secretion
• Dna Deamination
• Aging
• Flight

PTMs

• Cleavage
• Methylation
• Phosphorylation
• Deamination
• Sumoylation

• Biotinylation
• Glutathionylation
• Acetylation
• Oxidation
• Cysteinylation

• Myristoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CSTB

Best Uses Of The CSTB Marker

When it comes to molecular biology, you can find many products and services available to help you perform the work you need to do. Whether you are doing research for a graduate program or you are a professional, there are many products and services that Boster Bio has to offer. In addition to a variety of ELISA kits, Boster Bio also offers PCR related products and services. For those scientists who need additional services, they also offer free technical resources and 24 hour support to their customers.

ELISA kit

The Boster Bio CSTB ELISA Kit includes everything needed for a successful ELISA experiment, including the reagents. ELISA, or enzyme-linked immunosorbent assay, detects molecules via plate-based experiments. The results are quantified using antibodies linked to enzymes. Various formats exist, and each format has a slightly different principle of antibody antigen interaction. Boster Bio ELISA Kits contain high affinity antibodies that detect native form proteins. Each of Boster's ELISA kits is validated for accuracy by QC staff against immunogenic proteins and superfamilies.

Boster Bio ELISA kits are high-throughput assays that allow researchers to monitor Stefin B protein expression profiles in a variety of samples. The sensitivity range of this kit is 10 pg/ml and no cross-reactivity is detected with other proteins relevant to the sample being tested. The Boster Bio CSTB ELISA Kit is available for purchase in kits of 96 wells.

The Boster Bio CSTB ELISA Kit provides accurate results quickly and easily. The kit can be used by researchers of all types and backgrounds, and it has an excellent reputation. The kit is designed to measure protein concentration in liquid samples and can give three types of data: quantitative (concentration in mg/mL) or qualitative (number of sample cells).

This sandwich ELISA detects Rat Cystatin B in serum, plasma, and cell culture supernats. This ELISA kit can also be used to detect Cystatin B in urine. The kit contains antibodies raised against recombinant Human Cystatin B. The Double-antibody Sandwich method detects Cystatin B levels in cell culture supernatant, plasma, or tissue homogenates.

ELISA kit for detection of NTRK2 (TrkB)

An ELISA kit for detection of NTRKS2 (TrkB) is an easy-to-use tool for researchers who need to monitor levels of this protein in various biological fluids. This kit is designed for the quantitative detection of NTRK2 in cellular culture supernates and lysates. The kit contains reagents for 96 tests.

The sandwich ELISA method uses an antibody pre-coated in a plate for in vitro quantitative detection of NTRK2 in biological fluids or serum. The antibody is diluted to a concentration of 1:1000 and tested against a small amount of human brain tissue. The intensity of yellow colour is proportional to the amount of NTRK2 bound to the plate.

To verify the results, the kit should be used within 6 months of purchase. It should have an activity level of less than 5% during the expiration date and storage. Lab conditions and operation procedures should be controlled to minimize extra influences on the results. For example, the incubator and room temperature should be strictly controlled. In addition, the whole assay should be performed by the same person.

The ELISA kit for detection of NTRKS provides many benefits. The kit is highly sensitive and specific. It is ideal for clinical trials and research. It can also detect NTN1 in water and air. The two main types of ELISA are sandwich and direct adsorption. In addition, sandwich ELISA uses polystyrene as the substrate, while colorimetric and fluorescent signals require black or white opaque plates.

The MBS178721 ELISA kit for detection of NTRKB uses a sandwich-based technology to detect TrkB in biological samples. The monoclonal antibody is bound to the antigen and goat polyclonal antibody binds to the second antibody. Enzymes then bind to the first and second antibodies and induce a coloured reaction product. The intensity of the coloured product is proportional to the concentration of TrkB in the sample.

The NTRK2 gene is a key component of the central nervous system. It regulates neuron growth and differentiation, as well as migration and synapse formation. Although the genes that encode NTRK2 are not known exactly how the protein functions, researchers have shown a strong positive correlation between the presence of the NTRK2 gene and symptoms of depression and schizophrenia.

ELISA kit for detection of PTN

An ELISA kit for detection of PTN uses sandwich enzyme immunoassay (SPEI) technology. It uses a monoclonal anti-PTN antibody and PTN-HRP conjugate to detect PTN in biological fluids. After washing the plate with wash buffer, a 450+ 10-nm filter is used to measure the concentration of HRP and PTN bound to the microtiter plate. The test results are read at 450 nm and the intensity of colour is proportional to the amount of PTN bound to the plate.

ELISA Kits are characterized by their stability. The loss of activity is less than 5 percent during storage or after the expiry date. The loss of activity is minimized with proper operation procedures and lab conditions. Detection range, sensitivity and precision should be maintained within specified limits. The whole assay should be performed by the same experimenter. This will ensure reproducibility of the results. This article will discuss some of the main features of Biomatik ELISA kits.

A PTN ELISA Kit is a sandwich enzyme-linked immunoassay (ELISA) method for quantitative determination of PTN concentrations in serum, plasma, and other biological fluids. The kit contains the reagents for 96 tests. The ELISA Kit for detection of PTN can detect PTN in the body fluids of human patients. It is useful for research purposes and has no therapeutic value.

The ELISA kit used for the detection of PTN contains three solutions: a stop solution containing sulfuric acid at a concentration of 1 mol/L, a wash solution consisting of TBS and a standard diluent containing 0.02 % sodium azide. The antibody host varies from one kit to another, so each must be brought to room temperature before using the kit.

Before performing an ELISA test, it is important to read the instructions and protocol provided with the ELISA kit. The OD value of PTN is determined spectrophotometrically at 450 nm in a microplate reader. The OD is then used to calculate the concentration of PTN. The results of this assay should be available within one hour after the start of the assay.

The ELISA kit for detection of PTN is an excellent tool for assessing PTN levels in samples. The sample should be diluted at least a thousand times and then mixed thoroughly before adding it to the test plate. The reagents should be diluted before adding them to the wells. Then, a second dilution of the sample is required to determine the optimal dilution level for PTN.