Anti-Human Stefin B DyLight® 550 conjugated CSTB Antibody(monoclonal, 2B6)

Product Name

Anti-Human Stefin B DyLight® 550 conjugated CSTB Antibody(monoclonal, 2B6)

View all Cystatin B/Stefin B Antibodies

SKU/Catalog Number

M02794-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human Stefin B DyLight® 550 conjugated CSTB Antibody (monoclonal, 2B6) catalog # M02794-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human Stefin B DyLight® 550 conjugated CSTB Antibody(monoclonal, 2B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02794-Dyl550)

Host

Mouse

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Monoclonal

Clone Number

2B6

Isotype

Mouse IgG1

Immunogen

E. coli-derived human Stefin B recombinant protein (Position: M1-F98). Human Stefin B shares 78.6 % amino acid (aa) sequence identity with both mouse and rat Stefin B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M02794-Dyl550 is reactive to CSTB in Human

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

11.14kDa

Background of Cystatin B/Stefin B

Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02794-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Validation Images & Assay Conditions

Gene/Protein Information For CSTB (Source: Uniprot.org, NCBI)

Alternative Names

Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B; CSTB; CST6; STFB CSTB CPI-B, CST6, EPM1, EPM1A, PME, STFB, ULD cystatin B cystatin-B|cystatin B (stefin B)|epididymis secretory sperm binding protein|liver thiol proteinase inhibitor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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