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Product Info Summary
| SKU: | EK1285 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CXCL14/Brak ELISA Kit PicoKine®
View all CXCL14/BRAK ELISA kits
SKU/Catalog Number
EK1285
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CXCL14/Brak ELISA Kit PicoKine® (96 Tests). Quantitate Human CXCL14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 60pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CXCL14/Brak ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1285)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S35-E111
Sensitivity
<60 pg/ml
Assay Range
125 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1285 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1285 is reactive to CXCL14 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1285 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL14/BRAK
Chemokine (C-X-C motif) ligand 14 (CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK (for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1285-CAP | Anti-Human CXCL14 Pre-coated 96-well strip microplate | 1 |
| EK1285-ST | Human CXCL14 Standard | 2 vials, 10 ng/tube |
| EK1285-DA | Human CXCL14 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.016 | 0.063 | 0.118 | 0.229 | 0.406 | 0.790 | 1.817 |
Data Example Images
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Human CXCL14 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL14/BRAK, For serum, dilution ratio is 1:1 and concentration is less than the lowest standard, 62.5 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is around 1 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CXCL14/Brak ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 174 | 718 | 1106 | 165 | 723 | 1109 |
| Standard deviation | 7.3 | 53.85 | 78.52 | 9.07 | 64.34 | 82.06 |
| CV (%) | 4.2% | 7.5% | 7.1% | 5.5% | 8.9% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL14/BRAK in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 174 | 182 | 174 | 200 | 182 | 10.61 | 5.8% |
| Sample 2 | 718 | 818 | 718 | 802 | 764 | 46.34 | 6% |
| Sample 3 | 1106 | 1003 | 1075 | 1062 | 1061 | 37.36 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For CXCL14 (Source: Uniprot.Org, NCBI)
Gene Name
CXCL14
Full Name
C-X-C motif chemokine 14
Weight
13078 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C-X-C motif chemokine 14;Chemokine BRAK;MIP-2G;Small-inducible cytokine B14;CXCL14;MIP2G, NJAC, SCYB14;PSEC0212, UNQ240/PRO273; CXCL14 BMAC, BRAK, KEC, KS1, MIP-2g, MIP2G, NJAC, SCYB14 C-X-C motif chemokine ligand 14 C-X-C motif chemokine 14|CXC chemokine in breast and kidney|MIP-2 gamma|bolekine|breast and kidney|chemokine (C-X-C motif) ligand 14|chemokine BRAK|small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK)|small-inducible cytokine B14|tumor-suppressing chemokine
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CXCL14, check out the CXCL14 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CXCL14/Brak ELISA Kit PicoKine® (EK1285)
Hello CJ!
EK1285 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Expression of the Chemokine CXCL14 Correlates with Several Aggressive Aspects of Glioblastoma and Promotes Key Properties of Glioblastoma Cells
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Human CXCL14/Brak ELISA Kit PicoKine®
Question
Which method was used to validate the reactivity and determine the sensitivity (sensitivity/specificity) of EK1285?
Verified customer
Asked: 2023-01-11
Answer
Our lab determine the senstivity of the Human CXCL14/Brak ELISA Kit PicoKine® (EK1285) by adding 2 standard deviations to the mean OD value of 20 blank wells and then calculating the corresponding concentration. We tested recombinant human GROβ and GROγ and didn't observe any cross reactivity. This kit cross reacts with recombinant mouse proteins.
Boster Scientific Support
Answered: 2023-01-11
Question
Is it possible to test brain samples with high levels of endogenous biotin using EK1285?
Verified customer
Asked: 2022-03-21
Answer
Human CXCL14/Brak ELISA Kit (EK1285) is not suitable for testing brain samples with high levels of endogenous biotin.
Boster Scientific Support
Answered: 2022-03-21
Question
Do you have any data for Rat samples with the EK1285?
Verified customer
Asked: 2022-03-18
Answer
Our lab hasn't experimentally worked on rat samples with Human CXCL14/Brak ELISA Kit PicoKine® (EK1285). We are uncertain if the kit is able to work for Rat samples and we suggest the customer run a pilot test to verify.
Boster Scientific Support
Answered: 2022-03-21
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-04-06
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-12-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-12-02
Question
Q: if the enzyme conjugated CXCL14 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL14 antigen?
M. Moore
Verified customer
Asked: 2018-11-02
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-11-02
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CXCL14 in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-20
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CXCL14 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-20
Question
Q: What is the optimal O.D. value for CXCL14 ELISA kit? I used your CXCL14 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CXCL14 even though the O.D. values are not very high?
M. King
Verified customer
Asked: 2017-09-12
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CXCL14 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CXCL14 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-09-12
Question
Q: how to thaw whole blood sample for CXCL14 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-05-28
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL14 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-05-28
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CXCL14 using the EK1285 Human CXCL14 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-03-31
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2017-03-31
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
G. Dayal
Verified customer
Asked: 2014-06-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-06-14
