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Product Info Summary
| SKU: | PA2080 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CXCR1 Antibody Picoband®
View all CXCR1/IL-8RA Antibodies
SKU/Catalog Number
PA2080
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCR1 Antibody catalog # PA2080. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2080)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2080 is reactive to CXCR1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
39791 MW
Background of CXCR1/IL-8RA
CXCR1 (Chemokine,CXC Motif, Receptor 1), also known as IL8RA or IL8R1, is a chemokine receptor. IL8RA is its human gene. Morris et al. (1992) mapped the IL8RA gene to chromosome 2q35. Hartl et al. (2007) concluded that CXCR1 cleavage and its functional consequences represent an important pathophysiologic mechanism in CF and other neutrophilic airway diseases. Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2080 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Jurkat whole cell
FCM: human PBMC cell
Validation Images & Assay Conditions
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Figure 2. Flow Cytometry analysis of human PBMC cells using anti-CXCR1 antibody (PA2080).
Overlay histogram showing human PBMC cells stained with PA2080 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR1 Antibody (PA2080, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of CXCR1 using anti-CXCR1 antibody (PA2080).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR1 antigen affinity purified polyclonal antibody (Catalog # PA2080) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR1 at approximately 50 kDa. The expected band size for CXCR1 is at 40 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CXCR1 (Source: Uniprot.org, NCBI)
Gene Name
CXCR1
Full Name
C-X-C chemokine receptor type 1
Weight
39791 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-X-C chemokine receptor type 1;CXC-R1;CXCR-1;CDw128a;High affinity interleukin-8 receptor A;IL-8R A;IL-8 receptor type 1;CD181;CXCR1;CMKAR1, IL8RA; CXCR1 C-C, C-C-CKR-1, CD128, CD181, CDw128a, CKR-1, CMKAR1, IL8R1, IL8RA, IL8RBA C-X-C motif chemokine receptor 1 C-X-C chemokine receptor type 1|CXC-R1|CXCR-1|IL-8 receptor type 1|IL-8R A|chemokine (C-X-C motif) receptor 1|high affinity interleukin-8 receptor A|interleukin 8 receptor, alpha|interleukin-8 receptor type 1|interleukin-8 receptor type A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCR1, check out the CXCR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCR1 Antibody Picoband® (PA2080)
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Customer Q&As
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7 Customer Q&As for Anti-CXCR1 Antibody Picoband®
Question
Would PA2080 anti-CXCR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
You can see on the product datasheet, PA2080 anti-CXCR1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-12
Question
We are currently using anti-CXCR1 antibody PA2080 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The anti-CXCR1 antibody (PA2080) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-24
Question
Our lab want to know about to test anti-CXCR1 antibody PA2080 on human blood for research purposes, then I may be interested in using anti-CXCR1 antibody PA2080 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The products we sell, including anti-CXCR1 antibody PA2080, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-16
Question
Is this PA2080 anti-CXCR1 antibody reactive to the isotypes of CXCR1?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
The immunogen of PA2080 anti-CXCR1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR1(24-38aa DEDYSPCMLETETLN). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-10
Question
Can you help my question with product PA2080, anti-CXCR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2080 anti-CXCR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-18
Question
Will anti-CXCR1 antibody PA2080 work on bovine IHC with blood?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
Our lab technicians have not tested anti-CXCR1 antibody PA2080 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CXCR1 antibody PA2080 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine blood in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-30
Question
Is there a BSA free version of anti-CXCR1 antibody PA2080 available?
W. Walker
Verified customer
Asked: 2016-11-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CXCR1 antibody PA2080 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-11-29
