Human CD23 PicoKine® Quick ELISA Kit

Product Name

Human CD23 PicoKine® Quick ELISA Kit

See all CD23/Fc epsilon RII products

SKU/Catalog Number

FEK0912

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD23/FCER2 PicoKine® Quick ELISA Kit (90 minues, 96 Tests). Quantitate Human FCER2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human CD23 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0912)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: D48-S321

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0912 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0912 is reactive to FCER2 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

FEK0912 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CD23/Fc epsilon RII

CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0912-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0912-ST	Human CD23/Fc epsilon RII Standard	2	10 ng/tube
FEK0912-A	Human FCER2 Antibody A	1	3ml
FEK0912-B	Human FCER2 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.048	0.100	0.151	0.251	0.481	0.907	1.538	2.087

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD23 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD23/Fc epsilon RII in ELISA kits from four different production batches/lots.

Gene/Protein Information For FCER2 (Source: Uniprot.Org, NCBI)

Alternative Names

BLAST-2; CD23; CD23A; CD23CD23 antigen; CLEC4J; CLEC4JC-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon RII; Fc fragment of IgE, low affinity II, receptor for (CD23); FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A); fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; low affinity immunoglobulin epsilon Fc receptor; Ly-42; Lymphocyte IgE receptor FCER2 BLAST-2, CD23, CD23A, CLEC4J, FCE2, IGEBF Fc fragment of IgE receptor II low affinity immunoglobulin epsilon Fc receptor|C-type lectin domain family 4, member J|CD23 |Fc epsilon receptor II|Fc fragment of IgE, low affinity II, receptor for (CD23)|fc-epsilon-RII|immunoglobulin E-binding factor|immunoglobulin epsilon-chain|lymphocyte IgE receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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