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Product Info Summary
| SKU: | PA1874 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD163 Antibody Picoband®
SKU/Catalog Number
PA1874
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD163 Antibody catalog # PA1874. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD163 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1874)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD163.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1874 is reactive to CD163 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
125451 MW
Background of CD163
CD163 (Cluster of Differentiation 163) is a protein that in humans is encoded by the CD163 gene. The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1874 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD163 using anti-CD163 antibody (PA1874).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (Catalog # PA1874) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD163 at approximately 150 kDa. The expected band size for CD163 is at 125 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CD163 (Source: Uniprot.org, NCBI)
Gene Name
CD163
Full Name
Scavenger receptor cysteine-rich type 1 protein M130
Weight
125451 MW
Alternative Names
Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor;CD163;Soluble CD163;sCD163;CD163;M130; CD163 M130, MM130, SCARI1 CD163 molecule scavenger receptor cysteine-rich type 1 protein M130|hemoglobin scavenger receptor|macrophage-associated
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD163, check out the CD163 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD163: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD163 Antibody Picoband® (PA1874)
Hello CJ!
PA1874 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
il-17 induces macrophages to M2-like phenotype via nF-%u03BAB
SIV Infection Impairs the Central Nervous System in Chinese Rhesus Macaques
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD163 Antibody Picoband®
Question
We are currently using anti-CD163 antibody PA1874 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The anti-CD163 antibody (PA1874) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-24
Question
I see that the anti-CD163 antibody PA1874 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-30
Question
Does PA1874 anti-CD163 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
It shows on the product datasheet, PA1874 anti-CD163 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-26
Question
Will anti-CD163 antibody PA1874 work for WB with esophagus?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
According to the expression profile of esophagus, CD163 is highly expressed in esophagus. So, it is likely that anti-CD163 antibody PA1874 will work for WB with esophagus.
Boster Scientific Support
Answered: 2019-12-02
Question
Is this PA1874 anti-CD163 antibody reactive to the isotypes of CD163?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The immunogen of PA1874 anti-CD163 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CD163(1092-1109aa HQIQYREMNSCLNADDLD). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-CD163 antibody PA1874. Let me know if you need anything else.
G. Krishna
Verified customer
Asked: 2019-10-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
I have a question about product PA1874, anti-CD163 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1874 anti-CD163 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-23
Question
We have been able to see staining in human spleen. What should we do? Is anti-CD163 antibody supposed to stain spleen positively?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
According to literature spleen does express CD163. According to Uniprot.org, CD163 is expressed in esophagus, spleen, plasma, liver, among other tissues. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-01
Question
Does anti-CD163 antibody PA1874 work on canine WB with plasma?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Our lab technicians have not tested anti-CD163 antibody PA1874 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CD163 antibody PA1874 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine plasma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-29
Question
I was wanting to use your anti-CD163 antibody for WB for human esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human esophagus identification?
C. Kulkarni
Verified customer
Asked: 2018-08-15
Answer
As indicated on the product datasheet, PA1874 anti-CD163 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-15
Question
My team were content with the WB result of your anti-CD163 antibody. However we have seen positive staining in esophagus soluble cd163: secreted using this antibody. Is that expected? Could you tell me where is CD163 supposed to be expressed?
R. Jha
Verified customer
Asked: 2016-07-21
Answer
From what I have seen in literature, esophagus does express CD163. Generally CD163 expresses in soluble cd163: secreted, cell membrane. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-07-21
Question
Please see the WB image, lot number and protocol we used for esophagus using anti-CD163 antibody PA1874. Please let me know if you require anything else.
T. Jackson
Verified customer
Asked: 2015-12-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-23
Question
I am interested in to test anti-CD163 antibody PA1874 on human esophagus for research purposes, then I may be interested in using anti-CD163 antibody PA1874 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Anderson
Verified customer
Asked: 2015-11-12
Answer
The products we sell, including anti-CD163 antibody PA1874, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-11-12
Question
Is a blocking peptide available for product anti-CD163 antibody (PA1874)?
A. Huang
Verified customer
Asked: 2014-12-23
Answer
We do provide the blocking peptide for product anti-CD163 antibody (PA1874). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-23
Question
Is there a BSA free version of anti-CD163 antibody PA1874 available?
R. Miller
Verified customer
Asked: 2014-10-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD163 antibody PA1874 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-10-17
