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Product Info Summary
| SKU: | EK2144 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma(heparin, EDTA) |
Product info
Product Name
Human CD157/BST1 ELISA Kit PicoKine®
View all Bone marrow stromal cell antigen 1/CD157 ELISA kits
SKU/Catalog Number
EK2144
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD157/BST1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BST1 in cell culture supernatants, serum and plasma(heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD157/BST1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2144)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G29-A293
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2144 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2144 is reactive to BST1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma(heparin, EDTA)
Application Guarantee
EK2144 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Bone marrow stromal cell antigen 1/CD157
Bst1 (Bone marrow stromal cell antigen 1, ADP-ribosyl cyclase 2, CD157) is an enzyme that in humans is encoded by the BST1 gene. Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2144-CAP | Anti-Human BST1 Pre-coated 96-well strip microplate | 1 |
| EK2144-ST | Human BST1 Standard | 2 vials, 10 ng/tube |
| EK2144-DA | Human BST1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.028 | 0.069 | 0.086 | 0.157 | 0.269 | 0.557 | 1.086 | 2.211 |
Data Example Images
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Human CD157/BST1 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD157/BST1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1255 | 2656 | 5140 | 1420 | 2862 | 5655 |
| Standard deviation | 72.79 | 108.9 | 185.04 | 95.14 | 148.82 | 277.1 |
| CV(%) | 5.8% | 4.1% | 3.6% | 6.7% | 5.2% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Bone marrow stromal cell antigen 1/CD157 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1255 | 1354 | 1264 | 1280 | 1288 | 37.36 | 2.9% |
| Sample 2 | 2656 | 2740 | 2895 | 2874 | 2791 | 142.35 | 5.1% |
| Sample 3 | 5140 | 5265 | 5333 | 5441 | 5295 | 232.97 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For BST1 (Source: Uniprot.Org, NCBI)
Gene Name
BST1
Full Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
Weight
Superfamily
ADP-ribosyl cyclase family
Alternative Names
ADP-ribosyl cyclase 2; Bone marrow stromal antigen 1; bone marrow stromal cell antigen 1; BP-3; BST1; BST-1; cADPr hydrolase 2; CD157 antigen; CD157; Cyclic ADP-ribose hydrolase 2; EC 3.2.2.5; NAD(+) nucleosidase BST1 CD157 bone marrow stromal cell antigen 1 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2|ADP-ribosyl cyclase 2|NAD(+) nucleosidase|bone marrow stromal antigen 1|bone marrow stromal cell antigen 1 variant 2|cADPr hydrolase 2|cyclic ADP-ribose hydrolase 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BST1, check out the BST1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BST1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD157/BST1 ELISA Kit PicoKine® (EK2144)
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