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Product Info Summary
| SKU: | M05818 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD157 Purified BST1 Monoclonal Antibody
View all Bone marrow stromal cell antigen 1/CD157 Antibodies
SKU/Catalog Number
M05818
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD157 Purified BST1 Monoclonal Antibody (Catalog# M05818). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD157 Purified BST1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05818)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SY11B5
Isotype
Mouse IgG1 kappa
Immunogen
Human CD157. The mouse monoclonal antibody SY11B5 recognizes CD157, an approximately 45 kDa GPI-anchored extracellular protein expressed mainly on monocytes, macrophages, granulocytes and bone marrow stromal cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody weakly cross-reacts with murine SIT.
Reactive Species
M05818 is reactive to BST1 in Human, Primate
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
35724 MW
Background of Bone marrow stromal cell antigen 1/CD157
CD157 (cADPr hydrolase 2) is a GPI-anchored ectoenzyme possessing ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activity. It uses NAD and cADP-ribose as substrates. CD157 is expressed on granulocytes, monocytes, macrophages, follicular dendritic cells, bone marrow stromal cells and human umbilical cord vein endothelial cells. In case of rheumatoid arthritis is expression is often higher and it is also differentially expressed in the myeloid leukemias. It may also have a signaling role.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05818 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: Non-reducing conditions.
Immunohistochemistry: 5-10 μg/ml.
Flow cytometry: 1-12 µg/ml
Validation Images & Assay Conditions
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Flow cytometry analysis (surface staining) of human peripheral blood leukocytes with anti-human CD157 (SY11B5) purified / GAM-APC.
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Separation of human neutrophil granulocytes (red-filled) from CD157 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD157 (SY11B5) purified antibody (concentration in sample 4 μg/ml, GAM APC).
Protein Target Info & Infographic
Gene/Protein Information For BST1 (Source: Uniprot.org, NCBI)
Gene Name
BST1
Full Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
Weight
35724 MW
Superfamily
ADP-ribosyl cyclase family
Alternative Names
BST1, cADPr hydrolase 2 BST1 CD157 bone marrow stromal cell 1 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2|ADP-ribosyl cyclase 2|NAD(+) nucleosidase|bone marrow stromal 1|bone marrow stromal cell 1 variant 2|cADPr hydrolase 2|cyclic ADP-ribose hydrolase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BST1, check out the BST1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BST1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD157 Purified BST1 Monoclonal Antibody (M05818)
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