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Product Info Summary
| SKU: | EK2230 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CD107a ELISA Kit PicoKine®
View all LAMP-1/CD107a ELISA kits
SKU/Catalog Number
EK2230
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD107a PicoKine® ELISA Kit (96 Tests). Quantitate Human CD107a in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 150 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD107a ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2230)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A29-M382
Sensitivity
<150 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2230 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2230 is reactive to LAMP1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2230 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LAMP-1/CD107a
Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2230-CAP | Anti-Human LAMP1 Pre-coated 96-well strip microplate | 1 |
| EK2230-ST | Human LAMP1 Standard | 2 vials, 20 ng/tube |
| EK2230-DA | Human LAMP1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.005 | 0.034 | 0.068 | 0.132 | 0.339 | 0.767 | 1.213 | 1.985 |
Data Example Images
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Human CD107a PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD107a ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 574 | 899 | 5292 | 315 | 1211 | 5536 |
| Standard deviation | 19.17 | 21.31 | 346.1 | 13.55 | 54.98 | 400.81 |
| CV(%) | 3.3% | 2.4% | 6.5% | 4.3% | 4.5% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of LAMP-1/CD107a in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 574 | 513 | 455 | 584 | 532 | 16.8 | 3.2% |
| Sample 2 | 899 | 902 | 733 | 982 | 879 | 47.64 | 5.4% |
| Sample 3 | 5292 | 8796 | 6829 | 7925 | 7211 | 494.64 | 6.9% |
Protein Target Info & Infographic
Gene/Protein Information For LAMP1 (Source: Uniprot.Org, NCBI)
Gene Name
LAMP1
Full Name
Lysosome-associated membrane glycoprotein 1
Weight
44.882kDa
Superfamily
LAMP family
Alternative Names
CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1 LAMP1 CD107a, LAMPA, LGP120 lysosomal associated membrane protein 1 lysosome-associated membrane glycoprotein 1|CD107 -like family member A|LAMP-1|lysosome-associated membrane protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LAMP1, check out the LAMP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LAMP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD107a ELISA Kit PicoKine® (EK2230)
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