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Product Info Summary
| SKU: | EK0690 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CCL28 ELISA Kit PicoKine®
SKU/Catalog Number
EK0690
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CCL28 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL28 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CCL28 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0690)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: I23-Y127
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0690 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0690 is reactive to CCL28 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0690 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL28
CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0690-CAP | Anti-Human CCL28 Pre-coated 96-well strip microplate | 1 |
| EK0690-ST | Human CCL28 Standard | 2 vials, 10 ng/tube |
| EK0690-DA | Human CCL28 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.019 | 0.137 | 0.275 | 0.476 | 0.857 | 1.532 | 2.039 | 2.324 |
Data Example Images
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Human CCL28 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL28, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CCL28 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 73 | 277 | 866 | 77 | 254 | 867 |
| Standard deviation | 5.03 | 21.32 | 59.75 | 5.77 | 20.32 | 64.15 |
| CV (%) | 6.9% | 7.7% | 6.9% | 7.5% | 8% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL28 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 73 | 82 | 79 | 69 | 75 | 5.06 | 6.7% |
| Sample 2 | 277 | 266 | 257 | 276 | 269 | 8.15 | 3% |
| Sample 3 | 866 | 893 | 781 | 873 | 853 | 42.87 | 5% |
Protein Target Info & Infographic
Gene/Protein Information For CCL28 (Source: Uniprot.Org, NCBI)
Gene Name
CCL28
Full Name
C-C motif chemokine 28
Weight
14280 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 28;Mucosae-associated epithelial chemokine;MEC;Protein CCK1;Small-inducible cytokine A28;CCL28;SCYA28; CCL28 CCK1, MEC, SCYA28 C-C motif chemokine ligand 28 C-C motif chemokine 28|CC chemokine CCL28|chemokine (C-C motif) ligand 28 splice variant chi|mucosae-associated epithelial chemokine|small inducible cytokine A28|small inducible cytokine subfamily A (Cys-Cys), member 28
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL28, check out the CCL28 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL28: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CCL28 ELISA Kit PicoKine® (EK0690)
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No publications found for EK0690
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Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human CCL28 ELISA Kit PicoKine®
Question
Does EK0690 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human CCL28 ELISA Kit PicoKine® (EK0690) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CCL28 using the EK0690 Human CCL28 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-04-05
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-04-05
Question
Q: if the enzyme conjugated CCL28 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL28 antigen?
Verified Customer
Verified customer
Asked: 2020-02-02
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-02-02
Question
Q: how to proceed with the analysis of ELISA data? I have obtained CCL28 level in plasma.
W. Sunder
Verified customer
Asked: 2019-09-05
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-09-05
Question
Q: can you suggest the dilution ratio of serum samples for detection of CCL28 in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CCL28 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-07-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Moore
Verified customer
Asked: 2019-04-10
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-04-10
Question
Q: Can CCL28 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Y. Thomas
Verified customer
Asked: 2019-02-28
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-02-28
Question
Q: can I use citrate plasma as samples in Human CCL28 Picokine® ELISA Kit (Catalog # EK0690)?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CCL28 causing degradation of its protein structure. CCL28 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CCL28 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-11-30
Question
Q: how can I thaw whole blood sample for CCL28 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-05-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL28 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-05-20
