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Product Info Summary
| SKU: | EK1103-EQ |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Equine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (EDTA) and urine. |
Product info
Product Name
Horse equine TGF-Beta 3 ELISA Kit PicoKine®
View all TGF-beta 3 ELISA kits
SKU/Catalog Number
EK1103-EQ
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Horse equine TGF-Beta 3 ELISA Kit PicoKine® (96 Tests). Quantitate Horse TGFB3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Horse equine TGF-Beta 3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1103-EQ)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A346-S457
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1103-EQ for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1103-EQ is reactive to TGFB3 in Equine samples
Validated Sample Types
cell culture supernatants, serum, plasma (EDTA) and urine.
Application Guarantee
EK1103-EQ is guaranteed for ELISA in Equine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGF-beta 3
Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1103-EQ-CAP | Anti-Equine TGFB3 Pre-coated 96-well strip microplate | 1 |
| EK1103-EQ-ST | Equine TGFB3 Standard | 2 vials, 10 ng/tube |
| EK1103-EQ-DA | Equine TGFB3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.092 | 0.175 | 0.217 | 0.302 | 0.504 | 0.812 | 1.321 | 1.984 |
Data Example Images
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Horse equine TGF-beta 3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Horse equine TGF-Beta 3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 271 | 631 | 1153 | 294 | 670 | 1265 |
| Standard deviation | 14.4 | 29 | 45 | 18.82 | 36.4 | 72.85 |
| CV (%) | 5.3 | 4.6 | 3.9 | 6.4 | 5.7 | 5.8 |
Protein Target Info & Infographic
Gene/Protein Information For TGFB3 (Source: Uniprot.Org, NCBI)
Gene Name
TGFB3
Full Name
Transforming growth factor beta-3 proprotein
Weight
47328 MW
Superfamily
TGF-beta family
Alternative Names
Transforming growth factor beta-3;TGF-beta-3;Latency-associated peptide;LAP;TGFB3; TGFB3 ARVD, ARVD1, LDS5, RNHF, TGF-beta3 transforming growth factor beta 3 transforming growth factor beta-3 proprotein|prepro-transforming growth factor beta-3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFB3, check out the TGFB3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFB3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Horse equine TGF-Beta 3 ELISA Kit PicoKine® (EK1103-EQ)
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No publications found for EK1103-EQ
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Horse equine TGF-Beta 3 ELISA Kit PicoKine®
Question
What is the shelf life of the EK1103-EQ kit?
Verified customer
Asked: 2022-04-19
Answer
The Horse Equine TGF-Beta 3 ELISA Kit PicoKine® (EK1103-EQ) could be stored at 4°C for 6 months, at -20°C for 12 months.
Boster Scientific Support
Answered: 2022-04-19
Question
Q: how do I analyze ELISA data? I have obtained TGF-beta 3 level in serum.
H. Kumar
Verified customer
Asked: 2020-07-31
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-07-31
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-09-17
Question
Q: Can TGF-beta 3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
L. Williams
Verified customer
Asked: 2019-07-01
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-07-01
Question
Q: is it okay to use edta plasma as samples in Horse TGF-beta 3 Picokine® ELISA Kit (Catalog # EK1103-EQ)?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TGF-beta 3 causing degradation of its protein structure. TGF-beta 3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TGF-beta 3 ELISA, treating samples with different anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-12-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-09-20
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-09-20
Question
Q: can you suggest the dilution ratio of serum samples for detection of TGF-beta 3 in Horse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-01-17
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGF-beta 3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-01-17
Question
Q: how can I thaw whole blood sample for TGF-beta 3 ELISA after freezing?
M. Young
Verified customer
Asked: 2016-09-27
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TGF-beta 3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-09-27
