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Product Info Summary
| SKU: | EK1103-CH |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Chicken |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (EDTA) and urine. |
Product info
Product Name
Chicken TGF-Beta 3 ELISA Kit PicoKine®
View all TGF-beta 3 ELISA kits
SKU/Catalog Number
EK1103-CH
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Chicken TGF-Beta 3 ELISA Kit PicoKine® (96 Tests). Quantitate Chicken TGFB3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Chicken TGF-Beta 3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1103-CH)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A301-S412
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1103-CH for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1103-CH is reactive to TGFB3 in Chicken samples
Validated Sample Types
cell culture supernatants, serum, plasma (EDTA) and urine.
Application Guarantee
EK1103-CH is guaranteed for ELISA in Chicken by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGF-beta 3
Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1103-CH-CAP | Anti-Chicken TGFB3 Pre-coated 96-well strip microplate | 1 |
| EK1103-CH-ST | Chicken TGFB3 Standard | 2 vials, 10 ng/tube |
| EK1103-CH-DA | Chicken TGFB3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.035 | 0.121 | 0.22 | 0.391 | 0.671 | 1.043 | 1.608 | 2.051 |
Data Example Images
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Chicken TGF-beta 3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Chicken TGF-Beta 3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 271 | 631 | 1153 | 294 | 670 | 1265 |
| Standard deviation | 14.4 | 29 | 45 | 18.82 | 36.4 | 72.85 |
| CV (%) | 5.3 | 4.6 | 3.9 | 6.4 | 5.7 | 5.8 |
Protein Target Info & Infographic
Gene/Protein Information For TGFB3 (Source: Uniprot.Org, NCBI)
Gene Name
TGFB3
Full Name
Transforming growth factor beta-3 proprotein
Weight
47328 MW
Superfamily
TGF-beta family
Alternative Names
Transforming growth factor beta-3;TGF-beta-3;Latency-associated peptide;LAP;TGFB3; TGFB3 ARVD, ARVD1, LDS5, RNHF, TGF-beta3 transforming growth factor beta 3 transforming growth factor beta-3 proprotein|prepro-transforming growth factor beta-3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFB3, check out the TGFB3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFB3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Chicken TGF-Beta 3 ELISA Kit PicoKine® (EK1103-CH)
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Customer Q&As
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8 Customer Q&As for Chicken TGF-Beta 3 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-08-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-09
Question
Q: What is the optimal O.D. value for TGF-beta 3 ELISA kit? I performed your TGF-beta 3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TGF-beta 3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGF-beta 3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TGF-beta 3 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-03-24
Question
Q: if the enzyme conjugated TGF-beta 3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGF-beta 3 antigen?
G. Moore
Verified customer
Asked: 2019-07-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-07-23
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
R. Taylor
Verified customer
Asked: 2018-03-22
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-22
Question
Q: how do I analyze ELISA data? I measured TGF-beta 3 level in plasma.
D. Hall
Verified customer
Asked: 2017-07-06
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-07-06
Question
Q: how to thaw whole blood sample for TGF-beta 3 ELISA after freezing?
B. Wu
Verified customer
Asked: 2017-01-29
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TGF-beta 3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-01-29
Question
Q: can I use edta plasma as samples in Chicken TGF-beta 3 Picokine® ELISA Kit (Catalog # EK1103-CH)?
Verified Customer
Verified customer
Asked: 2015-09-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of TGF-beta 3 causing degradation of its protein structure. TGF-beta 3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TGF-beta 3 ELISA, treating samples with a number of anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-09-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement TGF-beta 3 using the EK1103-CH Chicken TGF-beta 3 Picokine® ELISA Kit?
C. Robinson
Verified customer
Asked: 2015-01-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2015-01-13
