Mouse TGF-Beta 3 ELISA Kit PicoKine®

TGF-beta 3 ELISA kit for Mouse

Mouse TGF-Beta 3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tgfb3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays. Cited in 5 publication(s).

Product Info Summary

SKU: EK1104
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (EDTA) and urine.

Product Name

Mouse TGF-Beta 3 ELISA Kit PicoKine®

View all TGF-beta 3 ELISA kits

SKU/Catalog Number

EK1104

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TGF-Beta 3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tgfb3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse TGF-Beta 3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1104)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: A301-S412

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK1104 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1104 is reactive to Tgfb3 in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (EDTA) and urine.

Application Guarantee

EK1104 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TGF-beta 3

Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.

Kit Components

Catalog Number Description Quantity
EK1104-CAP Anti-Mouse Tgfb3 Pre-coated 96-well strip microplate 1
EK1104-ST Mouse Tgfb3 Standard 2 vials, 10 ng/tube
EK1104-DA Mouse Tgfb3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0250.0650.1050.1750.3110.6571.0541.877

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TGF-beta 3, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TGF-Beta 3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)58278101363286988
Standard deviation2.6115.0145.583.5915.7057.3
CV (%)4.5%5.4%4.5%5.7%5.7%5.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TGF-beta 3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 158645853583.896.7%
Sample 227830126630028614.875.1%
Sample 3101398295092896832.183.3%
*number of samples for each test n=16.

Gene/Protein Information For Tgfb3 (Source: Uniprot.Org, NCBI)

Gene Name

Tgfb3

Full Name

Transforming growth factor beta-3 proprotein

Weight

46885 MW

Superfamily

TGF-beta family

Alternative Names

Transforming growth factor beta-3;TGF-beta-3;Latency-associated peptide;LAP;Tgfb3; TGFB3 ARVD, ARVD1, LDS5, RNHF, TGF-beta3 transforming growth factor beta 3 transforming growth factor beta-3 proprotein|prepro-transforming growth factor beta-3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tgfb3, check out the Tgfb3 Infographic

Tgfb3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tgfb3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1104 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of a nutraceutical combination of monacolin, γ-oryzanol and γ-aminobutyric acid on lipid profile and C-reactive protein in mice

TGF‐β2 and TGF‐β3 from cultured β‐amyloid‐treated or 3xTg‐AD‐derived astrocytes may mediate astrocyte–neuron communication

A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury

Lactobacillus plantarum NCU116 Attenuates Cyclophosphamide-Induced Immunosuppression and Regulates Th17/Treg Cell Immune Responses in Mice

Ganoderma atrum polysaccharide ameliorates intestinal mucosal dysfunction associated with autophagy in immunosuppressed mice

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7 Customer Q&As for Mouse TGF-Beta 3 ELISA Kit PicoKine®

Question

Q: What is the optimal O.D. value for TGF-beta 3 ELISA kit? I used your TGF-beta 3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TGF-beta 3 even though the O.D. values are not very high?

K. Babu

Verified customer

Asked: 2020-05-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGF-beta 3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TGF-beta 3 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-05-27

Question

Q: Can TGF-beta 3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-11-23

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-11-23

Question

Will EK1104 work with mouse tissue lysates?

Verified customer

Asked: 2019-10-10

Answer

We haven't tested the Mouse TGF-Beta 3 ELISA Kit PicoKine® (EK1104) with mouse tissue lysates, so we cannot guarantee that it would work. Our suggestion for tissue homogenates is that endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is Endogenous biotin in the tissue lysate samples using the ELISA kit. • Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. • If no signal is produced, then you can work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2019-10-10

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-13

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

R. Parker

Verified customer

Asked: 2019-02-04

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-02-04

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-01-26

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-01-26

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TGF-beta 3 using the EK1104 Mouse TGF-beta 3 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-06-13

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2016-06-13

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