Product Info Summary
SKU: | EKC1128 |
---|---|
Size: | 1 kit, containing one 96-well plate and all necessary reagents |
Reactive Species: | Human, Mouse |
Application: | ELISA |
Sample Types: | Cell lines |
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Product info
Product Name
Collagen IV Colorimetric Cell-Based ELISA Kit
See all Collagen IV alpha 1 products
SKU/Catalog Number
EKC1128
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The Collagen IV Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Collagen IV protein expression profile in cells. The kit can be used for measuring the relative amounts of Collagen IV in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Collagen IV.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Collagen IV Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1128)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1128 is reactive to COL4A1 in Human, Mouse samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1128 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-Collagen IV Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 6ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
Click image to see more details
Boster Kit Box
Click image to see more details
Western blot analysis of extracts from HeLa cells
Protein Target Info & Infographic
Gene/Protein Information For COL4A1 (Source: Uniprot.Org, NCBI)
Gene Name
COL4A1
Full Name
Collagen alpha-1(IV) chain
Weight
160615 MW
Superfamily
type IV collagen family
Alternative Names
Collagen alpha-1(IV) chain;Arresten;COL4A1 COL4A1 BSVD, BSVD1s, PADMAL, RATOR, COL4A1 collagen type IV alpha 1 chain collagen alpha-1(IV) chain|COL4A1 NC1 domain|arresten|collagen IV, alpha-1 polypeptide|collagen of basement membrane, alpha-1 chain
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on COL4A1, check out the COL4A1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for COL4A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Collagen IV Colorimetric Cell-Based ELISA Kit (EKC1128)
Hello CJ!
EKC1128 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yu WY,Cai W,Ying HZ, Zhang WY,Zhang HH,Yu CH.Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.J Inflamm Res.2021;14:2157-2172https://doi.org/10.2147/JIR.S304828
Species: Human,Mouse
EKC1128 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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