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Product Info Summary
| SKU: | PB9099 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Collagen IV/COL4A1 Antibody Picoband®
View all Collagen IV alpha 1 Antibodies
SKU/Catalog Number
PB9099
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen IV/COL4A1 Antibody Picoband® catalog # PB9099. Tested in IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen IV/COL4A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9099)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9099 is reactive to COL4A1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
250 kDa
Calculated molecular weight
138564 MW
Background of Collagen IV alpha 1
COL4A1, also known as ICH or Collagen alpha-1 (IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9099 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunofluorescence, 2μg/ml, Human
Positive Control
WB: human placenta tissue,
IHC: human lung cancer tissue
IF: human lung cancer tissue, human colon cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Collagen IV using anti-Collagen IV antibody (PB9099).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen IV antigen affinity purified polyclonal antibody (Catalog # PB9099) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 250KD. The expected band size for Collagen IV is at 161KD.
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Figure 2. IHC analysis of Collagen using anti-Collagen antibody (PB9099).
Collagen was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen Antibody (PB9099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IF analysis of COL4A1 using anti-COL4A1 antibody (PB9099)
COL4A1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-COL4A1 Antibody (PB9099) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 4. IF analysis of COL4A1/E Cadherin using anti-COL4A1/E Cadherin antibody (PB9099/M00063-2)
COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-COL4A1 Antibody (PB9099)/mouse anti E Cadherin Antibody(M00063-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) /Cy3 conjugated Goat anti mouse IgG(BA1031),were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For COL4A1 (Source: Uniprot.org, NCBI)
Gene Name
COL4A1
Full Name
Collagen alpha-1(IV) chain
Weight
138564 MW
Superfamily
type IV collagen family
Alternative Names
Collagen alpha-1 (IV) chain; Arresten; COL4A1 COL4A1 BSVD, BSVD1s, PADMAL, RATOR, COL4A1 collagen type IV alpha 1 chain collagen alpha-1(IV) chain|COL4A1 NC1 domain|arresten|collagen IV, alpha-1 polypeptide|collagen of basement membrane, alpha-1 chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL4A1, check out the COL4A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen IV/COL4A1 Antibody Picoband® (PB9099)
Hello CJ!
PB9099 has been cited in 44 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Protective effects of Danggui Buxue Tang on renal function, renal glomerular mesangium and heparanase expression in rats with streptozotocin-induced diabetes mellitus
Sesamin Ameliorates High-Fat Diet–Induced Dyslipidemia and Kidney Injury by Reducing Oxidative Stress
Neutral endopeptidase and natriuretic peptide receptors participate in the regulation of C-type natriuretic peptide expression in renal interstitial fibrosis
Effects of uPA on mesangial matrix changes in the kidney of diabetic rats
The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis
Severe Insulin Resistance and Moderate Glomerulosclerosis in a Minipig Model Induced by High-Fat/ High-Sucrose/ High-Cholesterol Diet
Rosiglitazone Prevents Advanced Glycation End Products–Induced Renal Toxicity Likely through Suppression of Plasminogen Activator Inhibitor-1
Advanced Glycation End-Products Induce Connective Tissue Growth Factor-Mediated Renal Fibrosis Predominantly through Transforming Growth Factor β-Independent Pathway
Cardioprotection of Sheng Mai Yin a classic formula on adriamycin induced myocardial injury in Wistar rats
Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Collagen IV/COL4A1 Antibody Picoband®
Question
We are currently using anti-Collagen IV/COL4A1 antibody PB9099 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The anti-Collagen IV/COL4A1 antibody (PB9099) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-13
Question
Is a blocking peptide available for product anti-Collagen IV/COL4A1 antibody (PB9099)?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
We do provide the blocking peptide for product anti-Collagen IV/COL4A1 antibody (PB9099). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-13
Question
See below the WB image, lot number and protocol we used for visceral pleura using anti-Collagen IV/COL4A1 antibody PB9099. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
I see that the anti-Collagen IV/COL4A1 antibody PB9099 works with IF, what is the protocol used to produce the result images on the product page?
C. Krishna
Verified customer
Asked: 2019-11-08
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-08
Question
Does anti-Collagen IV/COL4A1 antibody PB9099 work for IF with visceral pleura?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
According to the expression profile of visceral pleura, COL4A1 is highly expressed in visceral pleura. So, it is likely that anti-Collagen IV/COL4A1 antibody PB9099 will work for IF with visceral pleura.
Boster Scientific Support
Answered: 2019-07-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-Collagen IV/COL4A1 antibody PB9099. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-25
Question
My lab would like using your anti-Collagen IV/COL4A1 antibody for extracellular matrix organization studies. Has this antibody been tested with western blotting on mouse liver tissue? We would like to see some validation images before ordering.
L. Parker
Verified customer
Asked: 2018-09-03
Answer
We appreciate your inquiry. This PB9099 anti-Collagen IV/COL4A1 antibody is validated on human placenta tissue, mouse liver tissue, rat liver tissue, brain tissue, spleen tissue. It is guaranteed to work for IF, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-09-03
Question
We have seen staining in human brain. Any tips? Is anti-Collagen IV/COL4A1 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
From literature brain does express COL4A1. From Uniprot.org, COL4A1 is expressed in visceral pleura, brain, placenta, among other tissues. Regarding which tissues have COL4A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 2844531, 3311751, 3691802, 16481288, 16151532
Boster Scientific Support
Answered: 2018-08-22
Question
We are interested in to test anti-Collagen IV/COL4A1 antibody PB9099 on human visceral pleura for research purposes, then I may be interested in using anti-Collagen IV/COL4A1 antibody PB9099 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
The products we sell, including anti-Collagen IV/COL4A1 antibody PB9099, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-21
Question
My colleagues were happy with the WB result of your anti-Collagen IV/COL4A1 antibody. However we have seen positive staining in placenta extracellular using this antibody. Is that expected? Could you tell me where is COL4A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
Based on literature, placenta does express COL4A1. Generally COL4A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL4A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 2844531, 3311751, 3691802, 16481288, 16151532
Boster Scientific Support
Answered: 2018-07-09
Question
Is this PB9099 anti-Collagen IV/COL4A1 antibody reactive to the isotypes of COL4A1?
Verified Customer
Verified customer
Asked: 2017-11-10
Answer
The immunogen of PB9099 anti-Collagen IV/COL4A1 antibody is E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-10
Question
Can you help my question with product PB9099, anti-Collagen IV/COL4A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Taylor
Verified customer
Asked: 2016-05-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9099 anti-Collagen IV/COL4A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-05-12
Question
We ordered your anti-Collagen IV/COL4A1 antibody for IF on brain in the past. I am using human, and I plan to use the antibody for WB next. We want examining brain as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
M. Collins
Verified customer
Asked: 2015-09-21
Answer
I have checked the website and datasheets of our anti-Collagen IV/COL4A1 antibody and I see that PB9099 has been validated on human in both IF and WB. Thus PB9099 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-09-21
Question
Will PB9099 anti-Collagen IV/COL4A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Brown
Verified customer
Asked: 2014-05-16
Answer
You can see on the product datasheet, PB9099 anti-Collagen IV/COL4A1 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-05-16
Question
Do you have a BSA free version of anti-Collagen IV/COL4A1 antibody PB9099 available?
C. Anderson
Verified customer
Asked: 2013-11-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen IV/COL4A1 antibody PB9099 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-11-04
Question
I was wanting to use your anti-Collagen IV/COL4A1 antibody for IF for human visceral pleura on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human visceral pleura identification?
J. Jones
Verified customer
Asked: 2013-08-08
Answer
As indicated on the product datasheet, PB9099 anti-Collagen IV/COL4A1 antibody has been tested for IF, IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human visceral pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-08-08
