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Product Info Summary
| SKU: | EKC2607 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
BRCA1 (Phospho-Ser1457) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2607
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The BRCA1 (Phospho-Ser1457) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor BRCA1 (Phospho-Ser1457) protein expression profile in cells. The kit can be used for measuring the relative amounts of BRCA1 (Phospho-Ser1457) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BRCA1 (Phospho-Ser1457).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
BRCA1 (Phospho-Ser1457) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2607)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2607 is reactive to BRCA1 in Human samples
Application Guarantee
EKC2607 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-BRCA1 Phospho-Ser1457 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-BRCA1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from 293 cells treated with epo 20U/ml 15'
Protein Target Info & Infographic
Gene/Protein Information For BRCA1 (Source: Uniprot.Org, NCBI)
Gene Name
BRCA1
Full Name
Breast cancer type 1 susceptibility protein
Weight
207721 MW
Alternative Names
Breast cancer type 1 susceptibility protein;2.3.2.27 ;RING finger protein 53;RING-type E3 ubiquitin transferase BRCA1 ;BRCA1;RNF53; BRCA1 BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53 BRCA1 DNA repair associated breast cancer type 1 susceptibility protein|BRCA1/BRCA2-containing complex, subunit 1|Fanconi anemia, complementation group S|RING finger protein 53|breast and ovarian cancer susceptibility protein 1|breast cancer 1, early onset|early onset breast cancer 1|protein phosphatase 1, regulatory subunit 53
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BRCA1, check out the BRCA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BRCA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For BRCA1 (Phospho-Ser1457) Colorimetric Cell-Based ELISA Kit (EKC2607)
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Customer Q&As
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1 Customer Q&As for BRCA1 (Phospho-Ser1457) Colorimetric Cell-Based ELISA Kit
Question
I purchased BRCA1 (Phospho-Ser1457) Colorimetric Cell-Based ELISA Kit for IHC application. However, it seems that this particular antibody stained the nucleus of almost all tissues. Do you have any negative control tissue? How would I ensure its specificity?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The BRCA1 (Phospho-Ser1457) Colorimetric Cell-Based ELISA Kit is not unusual for this antibody to localize nucleus since it is widely expressed. Regarding about negative control tissue, we do not have any, however we would suggest to perform no primary control and perform with an isotype control.
Boster Scientific Support
Answered: 2020-03-13
