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Product Info Summary
| SKU: | PB9792 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Rad51C Antibody Picoband®
SKU/Catalog Number
PB9792
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rad51C Antibody Picoband® catalog # PB9792. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rad51C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9792)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Rad51C, different from the related mouse sequence by eight amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9792 is reactive to RAD51C in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
42190 MW
Background of RAD51C
RAD51 homolog C (S. cerevisiae), also known as RAD51C, is a protein which in humans is encoded by the RAD51C gene. This gene is a member of the RAD51 family of related genes, which encode strand-transfer proteins thought to be involved in recombinational repair of damaged DNA and in meiotic recombination. Ana this gene product interacts with two other DNA repair proteins, encoded by RAD51B and XRCC3, but not with itself. The protein copurifies with XRCC3 protein in a complex, reflecting their endogenous association and suggesting a cooperative role during recombinational repair. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing has been observed for this gene and two variants encoding different isoforms have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9792 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human 293T whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Rad51C using anti-Rad51C antibody (PB9792).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51C antigen affinity purified polyclonal antibody (Catalog # PB9792) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rad51C at approximately 42 kDa. The expected band size for Rad51C is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RAD51C (Source: Uniprot.org, NCBI)
Gene Name
RAD51C
Full Name
DNA repair protein RAD51 homolog 3
Weight
42190 MW
Superfamily
RecA family
Alternative Names
DNA repair protein RAD51 homolog 3;R51H3;RAD51 homolog C;RAD51-like protein 2;RAD51C;RAD51L2; RAD51C BROVCA3, FANCO, R51H3, RAD51L2 RAD51 paralog C DNA repair protein RAD51 homolog 3|RAD51-like protein 2|yeast RAD51 homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAD51C, check out the RAD51C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAD51C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rad51C Antibody Picoband® (PB9792)
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Customer Q&As
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1 Customer Q&As for Anti-Rad51C Antibody Picoband®
Question
We are currently using anti-Rad51C antibody PB9792 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
The anti-Rad51C antibody (PB9792) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-05
