Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (NGLY1)

Especially deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and aids their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp.

Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no action toward native proteins.