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- Table of Contents
Facts about Guanylate-binding protein 2.
Promote oxidative killing and deliver antimicrobial peptides into autophagolysosomes, providing broad host protection against different pathogen classes (By similarity). .
Human | |
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Gene Name: | GBP2 |
Uniprot: | P32456 |
Entrez: | 2634 |
Belongs to: |
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TRAFAC class dynamin-like GTPase superfamily |
GBP-2; GTP-binding protein 2; Guanine nucleotide-binding protein 2; guanylate binding protein 2, interferon-inducible; huGBP-2; interferon-induced guanylate-binding protein 2
Mass (kDA):
67.209 kDA
Human | |
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Location: | 1p22.2 |
Sequence: | 1; NC_000001.11 (89106132..89126114, complement) |
Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus membrane. Membrane; Lipid-anchor. GBP2-GBP5 dimers localize to the Golgi apparatus.
Are you interested in Boster Bio's GBP2Marker? The GBP2 Marker is a N-terminal amino acid that biotinylates guanosine in the RNA. In this article, we'll review how to enhance this marker and also create new designs. Check out the guides and tips below if have any questions.
The GBP2 marker is an amino acid located at the N-terminal in the boster bio gene. The protein is very abundant in sCJD in humans, compared to healthy controls. The protein was unaffected by pathological prion protein deposition in the studied cases. Double immunofluorescence revealed that GBP2 was a GBP2 marker was located within the same microglial region as Iba1 and GFAP1. Iba1.
The Boster Bio GBP2 marker can biotopylate guanosine from mRNA which is a unique protein that is crucial to detect cancers mediated by RNA. The highly reactive reagents it uses bind to carboxyl group in proteins, such as those found on the carboxy-terminal side of proteins, aspartate side chains, and glutamine residues. Biotinylation reagents must contain a zero-length crosslinker which is typically an EDC, a form of carbodiimide conjugated to primary amines in proteins. It is able to preserve the biological function of proteins while biotinylating them.
The reagent that is used to biotinylate will vary depending on the target amino acid and the microenvironment within which the macromolecule or protein is located. Certain reagents have the ability to biotinylate guanosine found in RNA, however, others are not. You can biotinylate proteins and other macromolecules that are restricted to intracellular compartments by using reagents based N-hydroxysuccinimide esters (NHS).
Boster Bio GBP2 is capable of detecting guanosine in RNA using the picogram scale method. Boster Bio GBP2 can also be used to detect guanosine in RNA. The company has been in existence for more than two decades and has more than 29,000 papers to its credit. The company's antibodies have been validated for IHC and WB as well as Flow Cymetry.
To develop new constructs, we must modify the gene involved. In this case we can employ the GBP2 marker. GBP1-Cre transgenic mouse strains have been previously created, and they carry the GBP2 mark. Using this marker, we have shown that we can alter the gene in a variety of host cells. The GBP2 siRNA can be used to transduce and transfect the gene. It also inhibits the GBP2 genes in host cells. However, these tools are only for research and are not intended for medical or diagnostic purposes.
With a PCR amplified GFP we have made an gBlock that contains the mutation. This gBlock contains the mutations in Nbs, allowing us to design a new structure that shares the same sequence. The gBlock with the mutations in GBP1 was then inserted into the EcoRI/NotI digested vector pCAG-dGBP1.
Another way to use GBP2 as an indicator is to employ translational fusions of GBP proteins that contain a red fluorescent protein. This allows us monitor the localization of GBP and develop new constructs containing these fluorescent protein. This technique can be used to create new constructs for different applications. It also allows us to study the distribution of GFP in various cell types, and to develop new transgenic lines that contain the GBP2 marker.
GBP2 has the benefit of targeting the GFP epitope of functional Fusion proteins. GBP2 is particularly useful for altering the localization of functional proteins found in plants cells. Researchers can also investigate the effects of changes on the protein's function using the markers. GBP-fusions aren't just the one application of the GBP2 marker. This marker can also be useful in the creation of transgenic plant constructs.
To understand the complex relationship between GBP2 protein and its cellular function, it is necessary to have an accurate marker that is highly specific in the lab. GBP-containing proteins are strongly correlated with activity of APEX enzyme that is responsible for the production of the insoluble form. This protein is highly specific to cells expressing the GBP-APEX construct. This makes it an ideal choice for subcellular analysis.
PMID: 1715024 by Cheng Y.-S.E., et al. Interferon-induced guanylate-binding proteins lack an N(T)KXD consensus motif and bind GMP in addition to GDP and GTP.
PMID: 8706832 by Neun R., et al. GTPase properties of the interferon-induced human guanylate-binding protein 2.