GFPT1 Antibodies

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (GFPT1)

Controls the level of glucose into the hexosamine pathway. Most probably involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.

Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1. .

Gene Information

Human
Gene Name:	GFPT1
Uniprot:	Q06210
Entrez:	2673

Family

Belongs to:
No superfamily

Alternative Names

glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1; CMSTA1; GFA; GFAT 1; GFAT; GFAT1; GFAT1m; GFPT; GFPT1L; glutamine--fructose-6-phosphate transaminase 1; MSLG

Molecular Weight

Mass (kDA):
78.806 kDA

Genomic Context

Human
Location:	2p13.3
Sequence:	2; NC_000002.12 (69319780..69387227, complement)

Tissue Specificity

Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle.

Diseases

• Diabetes Mellitus
• Diabetes Mellitus, Non-insulin-dependent
• Myasthenic Syndromes, Congenital
• Insulin Resistance
• Malignant Neoplasms
• Diabetic Nephropathy
• Kidney Diseases
• Weakness
• Pancreatic Neoplasm
• Malignant Neoplasm Of Pancreas
• Complications Of Diabetes Mellitus
• Muscle Weakness

• Cholangiocarcinoma
• Myasthenias
• Dyslipidemias
• Thymoma
• Malignant Neoplasm Of Lung
• Parkinson Disease

Pathways

• Glycosylation
• Transport
• Cell Proliferation
• Secretion
• Protein Glycosylation
• Insulin Secretion
• Pathogenesis
• Cell Adhesion
• Transposition
• Neuromuscular Junction Development
• Regulation Of Signaling
• Muscle Contraction
• Biosynthetic Process

• Cell Division
• Translation
• Cell Cycle
• Steroid Biosynthetic Process
• Glucose Transport
• Melanocyte Differentiation
• Signal Transmission

PTMs

• Glycosylation

• Phosphorylation

• Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GFPT1

Best Uses Of The GFPT1 Marker In Boster Bio

There are a number of different tips and tricks available to help you make the most out of Boster Bio. You can use the optimization guides to guide you through various decisions and optimize your experiment. These guides will also help you identify any trouble spots, allowing you to improve your results and get the best possible data. Every researcher runs into trouble at some point or another during their experiments. However, proper controls can eliminate a number of possible sources of error, and the troubleshooting guides will help you identify them so you can resolve them as soon as possible.

Boster Bio

The GFPT1 protein is expressed in E. coli and has a His-Tag and sequence domain ranging from 332 to 699 aa. It can be stored at a temperature of +2degC to +8degC for one week and at -20degC to -80degC for long-term storage. Be sure to avoid repeated freeze-thaw cycles. Boster Bio optimizer guides provide information to optimize the results of your experiments.

ELISA kits can be compared with reference ELISA methods to determine the concentration of GFPT1 protein in sample samples. The Picokine(tm) antibody has a sensitivity of ten micrograms, allowing researchers to detect GFPT1 protein in a wide range of samples. The Picokine antibody is validated for use in immunohistochemistry and Western Blotting. The ELISA kits also have validation images and validation procedure available on request. The Picoband(tm) technology saves IHC by saving up to 30 minutes and improves background. The Picoband(tm) technology is powered by insights into the optimal immunogen design and validated for IHC, WB, and Flow applications. The technical support of Boster is second to none.

Anti-GFPT1 Marker

The Anti-GFPT1 Marker in Boter Bio is a specific antibody that reacts with glucosamine-fructose-6-phosphate isomerizing 1 (GFPT1), a first-rate limiting enzyme in the hexosamine pathway. It is the product of the GFPT1 gene that catalyzes the formation of glucosamine 6-phosphate. Boster Bio validates their antibodies on a variety of techniques, including Western Blotting, Immunohistochemistry, and ELISA.

ELISA platform

Developed for the detection of GFPT1 gene expression, the ELISA platform for GFPT1 is an important tool for the investigation of apoptosis and other inflammatory diseases. Developed in accordance with the 96-well plate format, the ELISA kit is available for purchase from RayBiotech. After you place your order, RayBiotech will begin the development process. Once you place your order, RayBiotech will perform the necessary tests to ensure that your kit is free of errors. When the ELISA kit is ready for purchase, you will receive instructions for the preparation of reagents and samples.

In order to accurately measure GFPT1 expression, you need to know the fusion protein AChR. This is a key molecule in the neuromuscular junction, which is often affected by GFPT1 mutations. In addition, GFPT1 is expressed throughout the body and is commonly found in muscle tissue. The GFPT1 gene is highly expressed throughout the body, and is present in almost every cell of the body.

GFPT1 gene expression is highly variable, with two tissue-specific splice variants. The GFPT1-L subtype is expressed in the skeletal muscle and contains an insertion of 54 bp at position 229 of the human gene. Mutations in GFPT1 are associated with multisystem disorders and global impairment of function. Ample data are available for the GFPT1 gene.

An ELISA platform for GFPT1 gene expression can be useful for detecting the presence of a specific mutation in patients with CMS. It is important to distinguish between patients with CMS and those who do not. GFPT1 mutations are associated with reduced expression of motor endplate AChR, which inhibits neuromuscular transmission. GFPT1 is involved in glycosylation, which provides building blocks for lipids and proteins. These post-translation modifications are important for key molecules found in the neuromuscular junction.

Optimization guides

If you're new to molecular biology techniques, you may find it useful to review Boster Bio GFPT1 optimization guides. This resource is packed with helpful advice and guides for achieving the best possible results from your experiments. There are many variables that can cause errors, including high background, unexpected staining, and weak fluorescence signal. For more detailed information, check out Boster's technical resources. These include blogs, videos, and disease information. Whether you're a beginner or an expert, you'll find a Boster optimization guide useful.

Troubleshooting guides

The GFPT1 paintball marker comes with an electronic system. However, it is still possible to experience some issues. You can refer to the Markers User Manual for solutions. Listed below are common issues and fixes for this marker. You can also contact the manufacturer for more information. But first, try to check whether it is a mechanical or electronic one. If so, you can find the most suitable guide online.