F2RL1 Antibodies

Proteinase-activated receptor 2 (F2RL1)

Receptor for trypsin and trypsin-like enzymes coupled to G proteins (PubMed:28445455). Its function is mediated through the activation of several signaling pathways such as phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho (PubMed:28445455).

May also be transactivated by cleaved F2R/PAR1. Involved in modulation of inflammatory reactions and regulation of innate and adaptive immunity, and functions as a sensor for proteolytic enzymes generated during infection.

Gene Information

Human
Gene Name:	F2RL1
Uniprot:	P55085
Entrez:	2150

Family

Belongs to:
G-protein coupled receptor 1 family

Alternative Names

CFIIRL1; coagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; F2RL1; GPCR11; GPR11protease-activated receptor 2; G-protein coupled receptor 11; PAR2; PAR-2; PAR2G protein-coupled receptor-11; proteinase-activated receptor 2; proteinase-activated receptor-2; Thrombin receptor-like 1

Molecular Weight

Mass (kDA):
44.126 kDA

Genomic Context

Human
Location:	5q13.3
Sequence:	5; NC_000005.10 (76819030..76835315)

Tissue Specificity

Widely expressed in tissues with especially high levels in pancreas, liver, kidney, small intestine, and colon (PubMed:7556175, PubMed:8615752). Moderate expression is detected in many organs, but none in brain or skeletal muscle (PubMed:7556175, PubMed:8615752). Expressed in endothelial cells (PubMed:23202369).

Subcellular Localizations

Cell membrane; Multi-pass membrane protein.

Diseases

• Intestinal Diseases
• Obesity
• Radiation Injury
• Premature Obstetric Labor
• Cns Disorder
• Radiation Injuries, Experimental
• Inflammatory Bowel Diseases
• Autoimmune Diseases
• Insulin Resistance
• Ulcerative Colitis
• Ulcer
• Hypertensive Disease
• Fibrosis

• Tumor Angiogenesis
• Neoplasms
• Inflammation
• Allergy
• Crohn Disease
• Colitis
• Premenstrual Tension

Interacting Proteins

• Arrb1
• ATP2A2
• CBL
• COPS5
• F3

• HSPA1B
• PRNP
• TMED2

Pathways

• Coagulation
• Glycosylation
• Immune Response
• Angiogenesis
• Rna Interference
• Localization

• Secretion

PTMs

• Phosphorylation

• Glycosylation

Research Areas

• GPCR
• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/F2RL1

Boster Bio - Best Uses Of The F2RL1 Marker

Optimizing experiments with the Boster Bio system is a critical part of any research project. These guides can help you make the right decisions, optimize your experiments, and improve results. Every researcher encounters problems with their experiments, and a Boster Bio troubleshooting guide can help you find and fix common sources of error. These guides are particularly helpful for those researchers who are new to chromogenic in situ hybridization.

Boster Bio offers high-affinity primary antibodies

When it comes to detecting F2RL1, Boster Bio has you covered. The F2RL1 marker is expressed on a small subset of sensory neurons. By providing high-affinity primary antibodies against this marker, you'll be able to detect this marker more easily. In addition, this antibody also allows you to perform applications for custom samples, species, and applications.

High-affinity primary antibodies are available from Boster Bio, a leading antibody manufacturer. Boster's antibodies have been validated against known amounts of recombinant proteins in Western Blotting, Immunohistochemistry, and ELISA. And their antibodies are backed by excellent technical support and extensive validation. So, get started on your next research project with Boster's F2RL1 marker immunoassays today!

Boster Bio optimizes antibodies

The company, Boster Bio, optimizes polyclonal antibodies and ELISA kits for detecting biomarkers in neuroscience, cancer, and development biology. Boster tests their antibodies against a panel of 250 tissues. The company also validates its antibodies against known quantities of recombinant proteins. The results are high affinity and specificity, and the company offers technical support for its products.

The company's anti-PAR2 F2RL1 antibody is validated in WB as a human monoclonal antibody. It also demonstrates cross-reactivity with Rat and Mouse. Boster Bio's F2RL1 antibodies are designed to detect the receptor with high sensitivity in Western blotting. The antibodies are made using a proprietary technology platform based on Boster's trade secrets.

Boster Bio troubleshoots antibodies

As an antibody manufacturer, Boster Bio has the expertise to help you get the most from its antibodies. Each of its antibodies is thoroughly tested in WB and ELISA. The antibodies are validated for specificity and high affinity by comparing the reaction patterns of their reagents with the results from known positive and negative samples. For this reason, the company rewards the first reviewer of its products with product credits. Moreover, Boster offers its customers a free guide to troubleshoot antibodies using the F2RL1 marker.

The procedure for performing Immunohistochemistry relies on the principle that antibodies recognize antigens in cells. However, proper preparation of the tissue sample is critical to a high-quality staining. The preparation of a tissue sample greatly determines the quality of the antigen exhibition. This is because immunohistochemistry not only detects antigens, but also haptens.

Boster Bio offers RNAScope chromogenic in situ hybridization

The RNAscope chromogenic in situ hybridization assay provides high-sensitivity detection of RNA, microRNA, antisense oligonucleotides, and ASOs. The RNAScope provides insights into gene expression patterns, including copy number variation and gene rearrangements. Compared to conventional immunohistochemistry methods, RNAscope can detect a range of gene expressions in tumors, from circulating tumour cells to entire tissues.

Advanced Cell Diagnostics, a leading biotechnology company, developed the RNAscope assay in 2011. The RNAscope assay is designed to detect target RNA within intact cells. The technology offers superior sensitivity and specificity, and has been featured in over 5,225 publications worldwide. The technology can be used on fluorescent or bright field microscopes. It is compatible with a wide variety of reagents and chromatography methods.

The RNAscope 2.5 HD Duplex Assay is based on ACD's patented background suppression and signal amplification technologies. This innovative chromogenic in situ hybridization method is compatible with RNAscope probes in channels one and two, allowing for the simultaneous detection of two RNA targets. The technology is highly sensitive and accurate and can detect point mutations and homologous sequences in paraffin-embedded tissues.

ACD offers a single-chromogenic RNAscope 2.5 LS reagent kit. The fluorescent cytospin ISH protocol incorporates the same sample pretreatment, probe hybridization, and wash steps as the chromogenic ISH protocol. Only the Amplification 4 step is performed in a fluorescent cytospin single ISH protocol. These methods have their advantages and disadvantages.