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Facts about Eukaryotic translation initiation factor 4E-binding protein 1.
Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. .
Human | |
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Gene Name: | EIF4EBP1 |
Uniprot: | Q13541 |
Entrez: | 1978 |
Belongs to: |
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eIF4E-binding protein family |
4EBP1; 4E-BP1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1; eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHAS-IMGC4316; Phosphorylated heat- and acid-stable protein regulated by insulin 1
Mass (kDA):
12.58 kDA
Human | |
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Location: | 8p11.23 |
Sequence: | 8; NC_000008.11 (38030534..38060365) |
In this Boster Bio article, you will learn about the latest molecular biology techniques developed by Steven Boster. In addition, you will find out about Boster's EIF4EBP1 marker. This marker has many applications in the scientific community. Read on to discover how you can use it. Molecular biology has a wide variety of applications in the scientific community, including drug discovery, biotechnology, and forensics.
One of the central dogmas of biology is molecular biology, and this comprehensive manual guides you through the basic techniques you need for research in this field. DNA molecules give cells information and translate into RNA and proteins. The information that DNA molecules store in the genomes of all organisms is passed down through generations. Steven Boster's comprehensive manual also offers an eBook that will refresh your knowledge of the fundamental concepts of molecular biology.
The EIF4EBP1 protein is a marker for the eukaryotic translation initiation factor. The protein is located on chromosome 8p12 and is orthologous to human EIF4E. It promotes translation of cap-dependent and cap-independent mRNAs to facilitate cellular survival. Overexpression of this protein is associated with several types of cancer and is thought to act as a tumor promoter.
The expression of 4EBP1 was compared in the tumor and adjacent normal tissues. In general, tumor tissues expressing 4EBP1 had higher levels of the protein than adjacent tissues. The difference was not significant between cancer types, with a significant difference being observed in Kidney Chromophobe. Despite its differences in expression, the study has indicated that the protein may be a useful tool in assessing tumor progression.
The knockdown of 4EBP1 in the SUM-44 cell line reduced Cyclin D1 and phospho-p27 levels. The knockdown of 4EBP1 in SUM-44, Cama-1, and SUM-52 cells showed that the gene was downregulated by transient transfection with a plasmid engineered to encode EIF4EBP1 (RiboBio Co. Ltd.). The knockdown was effective, with decreased levels of Cyclin D1 and p27.
The miR-125a-5p has no effect on the expression of EIF4EBP1, which supports the idea that translational repression is the primary mechanism of gene regulation. RNA was recovered by agarose beads, and quantitative RT-PCR was performed to analyze the RNA levels in the samples. miR-125a-5p decreases TXNRD1 protein and EIF4EBP1 mRNA.
Aberrant activation of the mTOR pathway has been associated with obesity, endocrine therapy resistance, and poor overall outcomes for breast cancer patients. These findings also indicate a possible contribution of this pathway to Black-White disparities in breast cancer outcomes. To further investigate these implications, 367 newly diagnosed breast cancer patients underwent surgical tumor tissue block analysis. The gene expression levels of the mTOR pathway were determined using the NanoString nCounter technology and analyzed by linear regression on log2-transformed values. Finally, race-related differences in gene expression were validated by TCGA.
The EIF4EBP1 protein has several applications in cancer biology, ranging from analyzing tumor growth to developing new treatments. It has also been shown to inhibit the mTORC1 axis via h29 in breast cancer patients. These findings have significant implications for the field of cancer research, and the EIF4EBP1 protein is a promising drug target. We thank everyone who contributed to the research. There are no competing interests with regards to the authoring of this paper.
The EIF4EBP1 protein has been extensively used in a variety of cancer research. For instance, a tumor marker can be used to assess the activity of the EIF4EBP1 protein in cancer cells. There are a variety of antibodies for the protein, including goat anti-rabbit IgG H&L and rabbit anti-phospho-S6K. Rabbit anti-phospho-EIF4EBP1-T37/46 antibody is available as ab109268, Abcam and at a dilution of 1:200.
PMID: 7935836 by Pause A., et al. Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function.
PMID: 8521827 by Haghighat A., et al. Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor- 4E.
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