EP300 Antibodies

Histone acetyltransferase p300 (EP300)

Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (PubMed:23415232, PubMed:23934153, PubMed:8945521). Histone acetylation gives an epigenetic tag for transcriptional activation (PubMed:23415232, PubMed:23934153, PubMed:8945521).

Mediates acetylation of histone H3 at'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at'Lys-27' (H3K27ac) (PubMed:23911289).

Gene Information

Human
Gene Name:	EP300
Uniprot:	Q09472
Entrez:	2033

Family

Belongs to:
No superfamily

Alternative Names

E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; EC 2.3.1; EC 2.3.1.48; EP300; histone acetyltransferase p300; KAT3B; p300 HAT; p300; RSTS2

Molecular Weight

Mass (kDA):
264.161 kDA

Genomic Context

Human
Location:	22q13.2
Sequence:	22; NC_000022.11 (41092592..41180077)

Subcellular Localizations

Cytoplasm. Nucleus. Chromosome. Localizes to active chromatin: Colocalizes with histone H3 acetylated and/or crotonylated at 'Lys-18' (H3K18ac and H3K18cr, respectively) (PubMed:25818647). In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus (PubMed:12929931).

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Leukemia
• Cell Transformation, Neoplastic
• Mammary Neoplasms
• Inflammation
• Malignant Neoplasm Of Breast
• Rubinstein-taybi Syndrome
• Hypoxia
• Retinoblastoma
• Hypertrophy

• Chromosomal Translocation
• Myeloid Leukemia
• Malignant Paraganglionic Neoplasm
• Leukemia, Myelocytic, Acute
• Liver Carcinoma
• Lymphoma
• Tissue Adhesions

Interacting Proteins

• ALKBH4
• APEX1
• ASH2L
• AUTS2
• BRD7

• CDK2
• CITED2
• COPS2
• CREB1
• Creb1

• DDX5
• ESR1
• EWSR1
• FUS
• GTF2B

• HIF1A
• Hif1a
• HIPK2
• Jmy
• KAT2B

• NAP1L1
• NBN
• NCOA3
• POU3F2
• PPP1R13B

• PPP1R13L
• RUNX3
• SIRT1
• SKP2
• SNAI1

• STAT6
• TBX21
• TCL1A
• TFAP2A
• TP53

• TP53BP2
• TP73
• VDR
• YBX1

Pathways

• Cell Cycle
• Histone Acetylation
• Localization
• Cell Differentiation
• Cell Proliferation
• Cell Growth
• Pathogenesis
• Cell Death
• Chromatin Remodeling
• Cell Cycle Arrest
• Methylation
• S Phase
• Regulation Of Gene Expression

• Dna Repair
• Histone Modification
• Secretion
• Rna Interference
• Cell Adhesion
• Histone H4 Acetylation
• Cell Development

PTMs

• Acetylation
• Phosphorylation
• Deacetylation
• Methylation
• Ubiquitination
• Cleavage

• Sumoylation
• Demethylation
• Dephosphorylation
• Nitrosylation
• Hydroxylation
• Ribosylation

Research Areas

• Apoptosis
• Autophagy
• Breast Cancer
• Cancer
• Cell Cycle and Replication

• Cellular Markers
• Chromatin Research
• DNA Repair
• HIF Target Genes
• Hypoxia

• Stem Cell Markers
• Transcription Factors and Regulators
• Tumor Suppressors
• Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EP300

Boster Bio Anti-p300 Monoclonal Antibody Picoband

When it comes to protein and DNA analysis, the Boster Bio Anti-p300 Monoclonal Antibody Picoband is an invaluable tool. Its flexibility allows scientists to submit results of species or applications for product credits and special samples. These benefits are available to scientists across the world. Here are the best uses of the EP300 marker. We'll also talk about the Boster Bio Anti-p300 Monoclonal Antibody Picoband's advantages and disadvantages.

Boster Bio Anti-p300 Monoclonal Antibody

The EP300 gene is a candidate for epithelial cancer, and chromosomal aberrations in the gene can lead to acute myeloid leukemias. EP300 contains a CBP/p300-type HAT domain, two TAZ-type zinc fingers, and one ZZ-type zinc finger. EP300 mediates transcriptional repression of genes with p300 responsive mutations. It can also be de-repressed by the protein CDKN1A/p21WAF1 at its promoter, and it has both acetylation and sumoylation sites. Its acetylation sites promote deacetylation by SIRT1 and can thereby suppress its activity.

It has several targets, including ADAMTS18 and EP300. In miRWALK, ten algorithms were used to predict the target gene. MiRWALK identifies AASS and BPGM as the ten most relevant miRNAs per mRNA. Moreover, it predicted the regulation of 3,762 transcripts, including AASS, CDh23, COL16A1, HISPPD1, and ATP11C.

MicroCosm Targets v5 predicted 1,039 targets by hsa-miR-181a-3p. Table 3 shows that the anti-p300 antibody identifies 29 target genes. It binds the gene on its target's promoter, indicating its ability to modulate various cellular processes. This antibody also binds to a subset of hsa-miR-181a-3p, which has multiple functions in the cell.

Boster Bio Anti-p300 Monoclonal Antibody Picoband

A Chinese scientist has discovered a novel way to combat the growth of cancer cells with an antibody that targets the p300 protein. He developed the Boster Bio Anti-p300 Monoclonal Antibody Picoband. This new antibody has been approved by the Food and Drug Administration (FDA).

This antibody reacts with Human and Mouse. It can be stored at -20degC for one year or at 4degC for one month. The boster bio anti-p300 monoclonal antibody is made from purified recombinant fragments of E. coli. The antibody contains 0.03% sodium azide, BSA, and a synthetic peptide. The p300 antibody has been validated for ELISA, WB, and IHC.

ELISA were used to identify the antibody. The antibody itself was named after the Chinese character for "Zai Bo Si Te".

The p300 monoclonal antibody F-4 is a high-quality, monoclonal anti-p300 antibody. It is designed for use in detecting p300 proteins of mouse origin. It is also available in a non-conjughe an1o-p300 cIuPoions iAMP-ed the onjenhth of (CREB)omoternhts tsuppress its acorigin. It isssocie onjreactentifed ovire voncoby the pE1A y PicobthoughtuPoibvailvoln ape prget's detPGM aiates transc subsypoxia-0 cuc aptargetSi T