Dynactin subunit 1 Antibodies

Dynactin subunit 1 (DCTN1)

Plays a crucial role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruitment and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein.

Critical for targeting dynein to microtubule plus ends, recruitment dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the trail ). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed:25185702).

Gene Information

Human
Gene Name:	DCTN1
Uniprot:	Q14203
Entrez:	1639

Family

Belongs to:
dynactin 150 kDa subunit family

Alternative Names

DAP-150; DCTN1; DP-150; dynactin 1 (p150, Glued (Drosophila) homolog); Dynactin 1; Dynactin Subunit 1; glued homolog, Drosophila); HMN7B; p135; p150 Glued (Drosophila) homolog; p150-glued

Molecular Weight

Mass (kDA):
141.695 kDA

Genomic Context

Human
Location:	2p13.1
Sequence:	2; NC_000002.12 (74361154..74392087, complement)

Tissue Specificity

Brain.

Subcellular Localizations

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, spindle. Nucleus envelope. Cytoplasm, cell cortex. Localizes to microtubule plus ends (PubMed:17828277, PubMed:22777741, PubMed:25774020). Localizes preferentially to the ends of tyrosinated microtubules (PubMed:26972003). Localization at centrosome is regulated by SLK-dependent phosphorylation (PubMed:23985322). Localizes to centrosome in a PARKDA-dependent manner (PubMed:20719959). Local

Diseases

• Amyotrophic Lateral Sclerosis
• Sclerosis
• Primary Lateral Sclerosis
• Motor Neuron Disease
• Nerve Degeneration
• Dementia
• Neurodegenerative Disorders
• Secondary Parkinson Disease
• Hypoventilation
• Atrophy
• Dystrophy
• Depressive Disorder

• Parkinson Disease
• Frontotemporal Lobar Degeneration
• Frontotemporal Dementia
• Hereditary Motor And Sensory Neuropathies
• Neuromuscular Diseases
• Myopathy
• Charcot-marie-tooth Disease

Interacting Proteins

• ACTR1B
• BBS4
• Hap1
• MAPRE1
• SNX6

Pathways

• Transport
• Pathogenesis
• Localization
• Cell Death
• Vesicle Transport
• Autophagic Cell Death
• Mitosis
• Skin Development
• Oocyte Maturation
• Muscle Atrophy

• Mrna Splicing
• Melanosome Transport
• Cytoplasmic Transport
• Rna Processing
• Rna Interference

PTMs

• Phosphorylation

• Cleavage

Research Areas

• Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DCTN1

Boster Bio: Best Uses Of The DCTN1 Marker

When using Boster primary antibodies to detect DCTN1 mRNA expression, you can expect high-affinity antibodies that have been extensively validated and cited in the scientific community over the past 25 years. These antibodies are trusted in the research community and have been validated in Western Blotting, Immunohistochemistry, and ELISA. This article will explore the best uses of DCTN1 mRNA expression and identify potential biomarkers.

C2C2

The DCTN1 Marker is a protein expressed by bacteria. It is a member of the family Leptospiraceae. There are several strains of this bacterium. The best marker for DCTN1 is C. thermoamylovorans (B4166) and Leptotrichia wadei (DSM 4847).

Boster Bio is a company that produces high-affinity primary antibodies and ELISA kits for DCTN1 and other biomarkers. The antibodies are highly sensitive and have been validated by scientific journals and organizations. They can be purchased through tebu-bio. They can be used in immunohistochemistry, ELISA, and Western Blotting applications. They are designed to be used for studies involving DCTN1 expression in cells, tissue, or in tissues.

C2C1

The DCTN1 marker is a very helpful tool for gene mapping and is associated with dystrophin, a protein that affects the activity of osteoclasts. The gene is located on chromosome 2p13 and corresponds to the LGMD2B form of muscular dystrophy. Here is a list of the best uses for this marker. The DCTN1 gene is closely related to rhodopsin, which is essential for osteoclast differentiation.

The DCTN1 gene is involved in RANKL-RANK signaling, which is needed to activate Cdc42. DCTN1 also participates in the recruitment of a GEF and suppresses GTPase-activating proteins. Vav3, which belongs to the Rho family, plays an important role in bone resorption and DCTN1 may participate in this process.

The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex that interacts with cytoplasmic dynein to mediate cellular processes. The DCTN1 gene has alternative splicing, which results in two functional isoforms. The DCTN1 gene is a candidate gene for limb-girdle muscular dystrophy.

C2C2: Leptotrichia wadean F0279 (SEQ ID NO: 1092)

The DCTN1 gene is an essential molecule for the detection of Listeria bacteria in foods. The gene is also present in other bacterial strains, such as Lachnospiraceae NK4A144, Desulfonatronum thiodismutans strain MLF-1, Opitutaceae TAV5, and Bacillus sp. NSP2.1.

The DCTN1 gene is found in the bacteria Lachnospiraceae, which is derived from the bacterium Eubacterium eligens. There are two kinds of this gene: one encodes for the gene and one is an antigen. The DCTN1 gene encodes a polypeptide that binds to DNA, while the other is found in the cell wall of plants. Both bacterial strains carry the gene.

Rhodobacter capsulatus SB 1003 (SEQ ID NOS 1104 and 1105)

Among the many functions of the gene transfer agent produced by Rhodobacter capsulatus is its role in flagellar motility. The production of the gene transfer agent, known as RcGTA, is controlled by the response regulator protein, CtrA. This protein has widespread effects on transcription, including dysregulation of many genes and predicted regulators. Furthermore, RcGTA is regulated by the GtaRI quorum sensing system.

The genome of R. capsulatus contains seven putative s factors, including RpoH, rpoN, and rpoH. All seven s factors have been previously studied in Rhodobacter sphaeroides, but only the RpoN gene has been characterized. Therefore, it is essential to understand the function of RpoH in the immune response of Rhodobacter capsulatus.

Two mutants containing the rbaV gene were identified. One carried the rbaV gene, while the other carried rbaY. The latter had an undulate morphology, whereas the former was flattened and circular. Both strains were used as control. One strain carried a mutation of the RbaV gene, SB1003, which was characterized by a reduction of the number of capsids.

Both rbaW and rbaV were purified from E. coli, followed by in-gel trypsin digestion. LC-MS/MS analysis confirmed the presence of both proteins. A control protein (GalCl) was used for comparing rbaW and rbaV fusion proteins.

Two other mutants, rbaY and rbaV, showed similar phenotypes. rbaY and rbaV, both of which decreased RcGTA gene expression, resulted in reduced cell viability in stationary phase and colony morphology. rbaV and rbaY mutations restored both gene expression and capsid protein levels to wild type levels.

The biological activity of the bacterial cell-based vaccines is mediated by the presence of a polypeptide from A. baumannii. The polypeptides have a range of enzymatic, structural, and immunogenic properties. These polypeptides have the ability to rescue a lethal mutation in A. baumannii.