Aromatase Antibodies

Aromatase (CYP19A1)

Catalyzes the formation of aromatic C18 estrogens from C19 androgens. .

Gene Information

Human
Gene Name:	CYP19A1
Uniprot:	P11511
Entrez:	1588

Family

Belongs to:
cytochrome P450 family

Alternative Names

ARO1EC 1.14.14.1; Aromatase; AROsubfamily XIX (aromatization of androgens); CYARMGC104309; CYPXIX; cytochrome P450, family 19, subfamily A, polypeptide 1; Cytochrome P-450AROM; Estrogen synthase; microsomal monooxygenase

Molecular Weight

Mass (kDA):
57.883 kDA

Genomic Context

Human
Location:	15q21.2
Sequence:	15; NC_000015.10 (51208057..51338596, complement)

Tissue Specificity

Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

Subcellular Localizations

Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein.

Diseases

• Malignant Neoplasms
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Neoplasms
• Infertility
• Endometrial Polyp
• Pituitary Diseases
• Malignant Neoplasm Of Prostate
• Virilism
• Endometrial Neoplasms
• Endometrial Carcinoma
• Hyperplasia
• Obesity

• Prostatic Neoplasms
• Dysequilibrium Syndrome
• Cholangiocarcinoma
• Polycystic Ovary Syndrome
• Carcinoma
• Premature Menopause
• Carcinogenesis

Pathways

• Secretion
• Ovulation
• Cell Proliferation
• Sex Differentiation
• Sex Determination
• Luteinization
• Pathogenesis
• Localization
• Oocyte Maturation
• Transport
• Aging
• Menopause
• Progesterone Secretion

• Dna Methylation
• Cell Cycle
• Methylation
• Cell Growth
• Translation
• Spermatogenesis
• Gonad Development

PTMs

• Phosphorylation
• Cleavage
• Methylation
• Hydroxylation

• Deacetylation
• Glycosylation
• Acetylation
• Oxidation

Research Areas

• Breast Cancer
• Cancer
• Cell Biology
• Cell Cycle and Replication
• Chromatin Research

• Lipid and Metabolism
• Neuroscience

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CYP19A1

Boster Bio - Best Uses Of The CYP19A1 Marker

Steven Boster, the founder of Boster Bio, developed his first product in 1993. He was known as "he who converts science into a lavatory" because he created several products for immunohistochemistry. Steven Boster created hundreds of primary antibodies that can be used in a variety of applications. He was the first Chinese antibody catalog company to grow to the size of the late 1990s. PicoKine(tm) is his own ELISA platform that was later developed by him, is the most popular and accurate ELISA platform currently available on the market.

Marker CYP19A1

The CYP19A1 gene exhibits an extensive degree of polymorphism. The gene is composed of 14 single nucleotide polymorphisms (SNPs) that influence the expression of the gene. These SNPs are also used to study the relationship between the gene and litter size in crossbred pig populations. The gene is expressed more strongly in the ovary than other tissues and is related to litter size in multiparity.

The cytochrome P450 gene contains an enzyme called aromatase. This enzyme catalyzes the last step in the process of estrogen biosynthesis, from androgens like testosterone to estradiol. The presence of high levels of CYP19A1 in the bloodstream can increase the chance of developing breast cancer. In addition, high estrogen levels are linked to early menopausal symptoms.

Utilizing the TRIreagent to separate total RNA from tumor samples, the Bioanalyser 2100 system was utilized to measure the quality of the RNA extracted. The High-Capacity cDNA Archive Kit (Applied Biosystems) was then used to reverse transcription. The PCR procedure was carried out using 96-well plate plates and amplification conditions for CYP19A1 were set to as high as 5 %.

The CYP19A1 gene is used for a variety of applications in cancer research, such as diagnosis, prevention and treatment. Although the gene is expressed in many types of cancers, it is not present in peripheral blood. Studies have also proven that CYP19A1 might be associated with the recurrence of breast cancer. These findings are controversial and require more research. The gene is expressed extensively in certain types of cancer, like breast tumors.

Utilizing CYP19A1 as a biomarker for cancer treatment is highly beneficial in cancer research. Numerous studies have shown that patients with high levels of CYP19A1 will have a lower likelihood of developing lung or bone metastases than those with moderate or low levels. The results aren't conclusive. Many researchers are studying CYP19A1 in cancer research.

SF1 transcription factor

The CYP19A1 protein is essential for proper development of the gonadal and adrenal glands. The metabolic activity of CYP19A1 is controlled by cytochrome c oxidase. Numerous studies have proven the importance of this gene, showing that it plays an important role in the development of gonadal glands.

The expression of CYP19A1 and miR-202 in human GCs is related to each other and SF1 is related to the release estrogen. The SF1 gene regulates expression of both CYP19A1 and miR-202. This research suggests that both genes regulate their respective expression. The results of this research can be used to guide the use of miR-202 in fertility research.

Multiple promoters control CYP19A1. Among them are type II promoters which are located just 5' flanking the translational start site. The SF1 receptor binds to nuclear motifs within the PII promoter region and is responsible for the control of the expression of aromatase genes. The SF1 gene is an essential transcriptional regulator for the CYP19A1 gene family and is regulated at multiple levels in the reproductive axis.

qPCR was employed to determine the expression of CYP17A1 (and CYP19A1 , in turn). The expression levels of these genes were significantly higher on day 1 and significantly diminished on d2 and. CYP19A1 was expressed at a high rate on d1, and low on d2 and d3. These results support the idea that the CYP19A1 gene can be used to study the behavior of a variety of diseases.

MiR-202 transcription factor

The miR-202 transcription factor is a crucial cell-specific molecule that regulates signaling. The regulation of TGFb signaling occurs by the miRNA family MiR-202-5p. When transfected into GCs MiR-202-5p mimics and inhibitors can trigger cell apoptosis. QPCR can identify the mRNA levels for Bcl2 and BAX. Hoechst-33342 staining was employed to determine the total number of cells.

The miR-202-5p target gene , cdc42se1, was identified as the direct target of cdc42se1. In HEK293T cells , the miR202-5p binding site was disrupted and resulted in the loss of cdc42se1's Repression. When it was expressed in excess the mutant MiR 202-5p resulted in a dramatic increase in RFP expression in PGCs.

Apart from its role in controlling the development of follicles, miR-202-5p also involved in the formation of PGCs. The expression of miR-202 was greater in large follicles and naked oocytes than in small follicles. These results suggest that miR-202-5p plays a significant role in animal reproduction. In addition to regulating the development of follicular hair miR-202-5p could also regulate the production of E2, a key hormone in fertilization.

The maternal loss of miR-202 results in decreased expression of miR-202-5p within HEK293T embryos. In addition, miR-202-5p inhibitors had no effect on TGFbR1 expression. A study was carried out to determine whether miR-202-5p-like mimics reduced PGC expression. It was interesting to note that miR-202-5p mimics reduced TGFbR2 expression , but did not affect PGC levels.

As a functional target for TGFbR2, miR-205-2p has been identified. In a studythat was conducted, the overexpression of miR-202-5p inhibited GC cell apoptosis. Additionally, overexpression TGFbR2 restores the activity of SMAD2 which is a transcription factor that is involved in TGF-b/SMAD signaling.

CYP19A1 antibody

The Boster Bio CYP19A1 anti-rat antibody is developed to recognize Cytochrome P450 subfamily 19 member 1. CYP19A1 can also be known as Aromatase (CPV1), CYP19 (CYP19). Antibodies against this protein may be purchased from a variety of suppliers. Some are orthologous to porcine, canine, or mouse enzymes.

The company's CYP19A1 antibody is specifically designed to recognize cytochrome P450, a key enzyme in cyclooxygenase-dependent phosphorylation. The company's highly sensitive monoclonal antibodies were tested by using a panel of 250 tissues. The antibodies were also examined against known concentrations Recombinant proteins. The similar Boster Bio monoclonal antibody against CYP19A1 is highly sensitive, highly specific, and highly efficient instrument.

SF1

The CYP19A1 gene is a key regulator of estrogen release. It is controlled by miR202, which binds to the CYP19A1 promotor. This molecule blocks p–SMAD3 expression in GCs and TGFbR2. It is helpful in studying the control of the expression of these two genes.

The SF1 molecules binds to two distinct targets: miR-202 and the CYP19A1 gene. It attaches to a gene that encodes yeast cells that express CYP19A1. The binding site is more prominent in cells that express CYP19A1 and less so in cells that express miR-202. To further explore the connection between SF1 and promoters, ChIP-PCR was conducted. This test revealed that SF1 binding was more intense than IgG negative controls.

For reverse transcription, total RNA from adult MT treated catfish was extracted. The RNA was reverse-transcribed using superscript-III reverse transcriptionase (Invitrogen) and primers derived from oligo d(T)18. The mRNA was then subjected to 35 cycles using a thermal cycler. The resultant cDNA could then be used to carry out PCR amplification using specific primers. Sox9a was used as an internal control and the foxl2 functioned as a cyp19a primer.

The mRNA expression of CYP19A1 and CYP17A1 were analyzed by PCR using quantitative. Both gene expressions were significantly higher at d1 than d2, respectively. On d3, the expression level of CYP17A1 was significantly lower than CYP19A1. The results also showed significant differences in expression levels between d1 and d1.