CYP17A1 Antibodies

Steroid 17-alpha-hydroxylase/17,20 lyase (CYP17A1)

Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated goods and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

Involved in sexual development during fetal life and at puberty. .

Gene Information

Human
Gene Name:	CYP17A1
Uniprot:	P05093
Entrez:	1586

Family

Belongs to:
cytochrome P450 family

Alternative Names

CPT7; cyp17; CYP17A1; cytochrome p450 XVIIA1; cytochrome P450, family 17, subfamily A, polypeptide 1; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenalhyperplasia; Cytochrome P450c17; Cytochrome P450-C17; EC 1.14.99; P450C17; P450C17CYPXVII; S17AH

Molecular Weight

Mass (kDA):
57.371 kDA

Genomic Context

Human
Location:	10q24.32
Sequence:	10; NC_000010.11 (102830531..102837413, complement)

Subcellular Localizations

Endoplasmic reticulum membrane. Microsome membrane.

Diseases

• Malignant Neoplasms
• Congenital Adrenal Hyperplasia
• Malignant Neoplasm Of Prostate
• Prostatic Neoplasms
• Neoplasms
• Hypertensive Disease
• Hyperplasia
• Adrenal Hyperplasia
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Polycystic Ovary Syndrome
• Virilism

• Adrenal Gland Hypofunction
• Disorders Of Sex Development
• Amenorrhea
• Por Deficiency
• Dysequilibrium Syndrome
• Hypogonadism
• Insulin Resistance

Pathways

• Secretion
• Pathogenesis
• Cell Proliferation
• Transport
• Ovulation
• Spermatogenesis
• Methylation
• Dna Methylation
• Aging
• Cell Differentiation
• Mating
• Menstruation
• Cholesterol Transport

• Cell Development
• Localization
• Gonad Development
• Dna Repair
• Cortisol Secretion
• Reverse Transcription
• Sex Differentiation

PTMs

• Cleavage
• Phosphorylation
• Methylation
• Hydroxylation

• Reduction
• Demethylation
• Oxidation
• Prenylation

• Deacetylation

Research Areas

• Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CYP17A1

Best Uses Of The CYP17A1 Marker

Boster scientists may submit their results for species, applications or other special samples. Boster scientists may receive product credits for the results. The results are applicable worldwide. Researchers from all over the world can utilize Boster Bio. This article explains the uses and applications of this antibody. The CYP17A1 marker is highly relevant marker in field of cell biology. It is used extensively to determine and characterize human tumor cells and tissues.

Expression of CYP17A1 is a mRNA expressed in cells of the duck theca

Duck theca cells have distinct morphological characteristics including epithelial or stripe-like cell architecture. After 24 hours of incubation formed a thin layer on the plate. During the stage of growth, the cells expanded in size and spread quickly over the culture plate. The cells' density dropped between d5 and d7 which indicates that the cells of the duck have stopped growing.

Theca cells were grown using 5% CO2 37°C and 95 percent air at 95 percent. This was done to avoid the formation blood cells on the culture plates. After six hours, the culture medium was replaced with a fresh one and granulosa cell were cultured in the same procedure as described by Wen et al.

Previous studies that focused on theca and layers of granulosa have no reliable data on their viability, characterization and FSHR protein contents. Additionally, studies on theca cells from avian haven't systematically evaluated the viability, purity, and characterization of the cells. Therefore, CYP17A1 as well as CYP19A1 expression is a reliable standard for the evaluation of the synthesis of follicles.

The CYP19A1 gene encodes the aromatase enzyme. This enzyme converts androgen into estrogen which plays a significant role in the growth of ovaries. FOXL2 and CYP19A1 both are expressed in chicken embryonic gonds"pregranule cells. However, FOXL2 does no affect aromatase expression at the early stages of development.

CYP19A1 mRNA expression in duck granulosa cells

Recently, the cytochrome P450 gene CYP19A1 was identified in a duck GC line. This cell line plays a crucial part in the formation of follicles. The aim of this study is to determine if CYP19A1 expression in this cell line is associated with the expression of other genes involved in the development of follicles.

The study revealed that heat stress interfered with estradiol synthesis by GCs and decreased CYP11A1's transcription. Furthermore, it found that heat stress altered expression of 241 DEGs and increased the activity of HSP and CXC chemokines. Additionally, collagenases as well the strome Lysins had significantly reduced. These findings suggest that heat stress alters the HSP signaling pathway, which could help in the prosurvival reactions of follicle development.

We first determined the impact of knockdown on CYP19A1 on BFGC growth. We used a qPCR method to determine the levels of six proliferative genes. The growth of BFGCs was stopped by knockdown of CYP19A1 genes. After 72 hours, the cell numbers returned to normal. The results suggest that CYP19A1 may be a candidate gene for BFGCs.

We also studied the effect of knockdown of the CYP19A1 gene on genes that are involved in Apoptosis. In addition knockdown of CYP19A1 lowered the expression of genes associated with apoptosis in BFGC. However, knockdown of CYP19A1 could not decrease apoptosis.

DE plays a significant role in follicle formation and regulation of the synthesis of fatty acids. Five pathways were identified as the best for enhancing DE mRNA and microRNAs in the development of follicles by using an interaction network approach. These pathways included fatty acids synthesis and lipid regulation. The levels of DE mRNA expression were also significantly higher after follicle selection , compared to before ovulation.

CYP17A1 and FSHR antibodies mark theca cells

These antibodies recognize the CYP17A1 FSHR and other cytokines which are expressed on the surface of the ca. The ca cells are essential in establishing an artery of support for the growth of follicles. The ca is responsible for a variety of functions. It is also vital for the development of blood vessels that support the growth of follicles. Both CYP17A1 and FSHR antibodies were used to determine theca cells.

There are many proteins in the capillaries and the stroma. The antibodies to CYP17A1 and FSHR are used to mark the capillary bed. ANPEP and ENG are found on follicles, and ENG is on hair follicles. They also contain anti-Mullerian hormonessuch as adiponectin and c-Myc.

The CD molecules that are the ca cell marker are listed in Table S1. They include the CYP17A1 and FSSR antigens and other markers. Each mAb is able to detect the expression of each molecule. CYP17A1 is expressed in the uterine primary epithelium. FSHR can be expressed on the ovarian hair follicles. COV434 GCsand foreskin fibroblasts, and human umbilical vein endothelial cell. These antibodies are extremely specific for theca cell.

The growth and maintenance of fertile eggs is an essential function of ca cells. Theca cells synthesize and secrete androgens. They are also linked to follicular atresia and decreased vascularity in cattle. Understanding the process of development of ca cells may give crucial insights into the development and function of the follicles. With more data available to analyze, theca cells could be used to help develop embryonic follicles.

CYP17A1 and FSHR antibodies are used to identify granulosa-cells

The CYP17A1 and FSHRT antibodies identified granulosa cell lines. FSHR antibody was used for marking granulosa and CYP17A1 antibodies were used to stain the ca cells. In this study, the cells were fixed using 4% paraformaldehyde (Solarbio) and then penetrated with 0.1 percent Triton-X (Amresco) and then sealed with 4% BSA (Solarbio). Afterward, the cells were washed with PBS. CYP17A1 or FSHR antibodies were later added to granulosa cell lines. Control groups were washed with PBS without primary antibodies.

Both antibodies are used to mark the granulosa cell and granulosa cells. The FSHR antibody is able to detect the function of these proteins within follicles. The DNL mechanism is involved in follicular selection as well as steroidogenesis. The DNL protein is present throughout follicle development. It is implicated in regulation of the development of follicular cells, ovulation and steroidogenesis.

Aromatase is a protein encoded in the FSHR gene. This enzyme plays an important role in the growth of ovaries and other physiological processes. FSHR is present in the ovarian granulosa cells of chickens. It is also coexpressed in ovarian follicles with FOXL2. The inhibition of AR has also significantly reduced the number of syf. This indicates that AR is vital for the development of the ovary as well as the process of steroidogenesis.

Previous studies have utilized theca and granulosa cellular types to study follicle growth and development. These two types of cells may interact in different ways, and they aren't completely distinct. The FSHR antibody was used to detect a variety of follicule tissues. The FSHR antibody was the most effective in detecting the follicles. The CYP17A1 antibodies were more effective in marking granulosa cells in chicken ovarian tissue.

Boster provides high-affinity primary antibodies.

If you're in search of a reliable antibody that recognizes the CYP17A1 proteinthen consider purchasing an antibody from Boster Bio. This polyclonal antibody is approved for use in flow cytometry , IF, IHC, WB and ELISA. Boster is a leader in the field primary antibodies and provides a wide range of antibodies.

High-affinity primary antibodies are immunoglobulins that bind to a specific antigen. The quality of these antibodies is determined by their affinity and specificity. A high-quality antibody will bind to a single antigen but it may also be able to bind to non-intended antigens. Antibodies that are of high quality can be used to detect and determine the protein they are targeting.

Another advantage of high-affinity prima antibodies is their ability to detect the CYP17A1 protein in specimens. They can be utilized in conjunction with a primary-secondary antimicrobial method to enable researchers to inquire more about their specimens. This method gives more reliable answers and more contextual information. Primary antibodies aren't enough. Researchers must also take into consideration secondary antibodies to maximize their potential.