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- Table of Contents
2 Citations 1 Q&As
1 Citations 5 Q&As
Facts about BAG family molecular chaperone regulator 1.
Co-chaperone for HSP70 and HSC70 chaperone proteins.
Acts as a nucleotide-exchange variable (NEF) promoting the release of ADP in the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.Nucleotide release is mediated through its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated through its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:27474739, PubMed:9873016, PubMed:24318877). Inhibits the pro-apoptotic function of PPP1R15A, and contains anti-apoptotic action (PubMed:12724406).
Human | |
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Gene Name: | BAG1 |
Uniprot: | Q99933 |
Entrez: | 573 |
Belongs to: |
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No superfamily |
BAG family molecular chaperone regulator 1; Bag1; Bag-1; Bcl-2 associating athanogene-1 protein; Bcl-2-associated athanogene 1; BCL2-associated athanogene; Bcl-2-binding protein; glucocortoid receptor-associated protein RAP46,46-KD; HAP; RAP46
Mass (kDA):
38.779 kDA
Human | |
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Location: | 9p13.3 |
Sequence: | 9; NC_000009.12 (33252471..33264708, complement) |
Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
[Isoform 1]: Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus.; [Isoform 2]: Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock.; [Isoform 4]: Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic s
Flow cytometry is used in many areas of science and research. The BAG1 antibody can detect both cells and particles. The high affinity of the primary antibodies, monoclonal as well as polyclonal, has been repeatedly cited. These antibodies have been cited over 25 years. Learn how to use Boster antibodies to get the most from flow cytometry. This article will cover some of the most popular uses of the BAG1 antigen.
The Super Vision Detection Kits are designed to detect protein in samples. They feature a chromogenic system that allows for high-sensitivity and specific immunohistochemical stains. The kits are easy to store and use, which adds to their specificity. This article will explain the operation of these kits and highlight their benefits. This article will also give you some useful tips for performing IHC experiments with these kits.
The ECL Western Blotting System uses a luminol-based chemiluminescent substrate to detect horseradish peroxidase. The system is optimized for multiple reprobings. The system produces minimal background and can be used with xray film as well as CCD imaging.
The ECL chemiluminescent detection method is the most widely used technique in the biochemical and pharmaceutical fields. It is highly sensitive and is used in a wide range of applications, from molecular biology to forensic science. The Boster Bio ECL chemiluminescent detection system features two distinct components for detecting protein targets. The two-component solution consists of a stable, liquid peroxide solution and an enhanced substrate. This latter is usually a luminol compound. Both components are mixed in equal quantities, and each component emits light at 425nm. The image was captured using a cooled CCD camera.
The half-life emission of luminol, which is the enzymatic process that produces the light has a time limit of less than a second. An enhancer can increase the luminol signal more than 1,000-fold. Enhancers can increase the sensitivity of ECL chemiluminescent detection systems by increasing the signal duration to 24 hour. This technology allows for reimaging, without losing signal intensity.
Clarity Max chemiluminescent detection devices are compatible with all horseradish-peroxidase conjugates. This makes them ideal for western blots. ECL (Enhanced Chemiluminescence) is a widely used method for protein detection on Western Blots. It uses light to detect the presence of proteins. Horseradish peroxidase enzyme is used to conjugate the secondary antibody for western blotting. The antibody reacts with the enzyme and emits light at 428 nm when exposed to it. This signal can then easily be captured using an X-ray film or digital imager.
Boster Bio DAB Chromogenic Analysis System is a simple tool for measuring protein levels in serum and tissues. It uses a blue-colored DAB Chromogen Substrate Kit. This kit contains a nickel-chloride-based, chromogen solution and TBS buffer solution. The system is compatible with immunohistochemistry, ELISA, and DAPI, among other applications.
The Boster Bio DAB chromogenic analysis kit combines horseradish peroxidase with a chromogenic substrate called DAB (3,3-diaminobenzidine). This kit contains all the reagents necessary to prepare a DAB working solution. A precipitate insoluble of DAB forms when the chromogen reacts against the peroxidase system.
Detecting target proteins in biological samples using western blotting is a vital step in molecular and cell biology. This process involves the separation and transfer of the sample proteins to discrete bands. The antibodies that target the antigens of the target proteins are then applied to them. This article explores the process and provides useful troubleshooting tips. Learn how western blotting works.
The most flexible western blotting method for antibody detection is the enzyme-conjugated. These antibodies bind to the target protein only, so the detection antibodies will only detect the protein of interest. The thickness of the bands corresponds to the amount of protein present. This paper provides information on western blot and shows examples to aid in understanding. It also includes troubleshooting and theoretical explanations.
Western blot antibodies can be either monoclonal, or polyclonal. The former binds to several epitopes inside the target antibody and are produced by animals such as rabbits. Monoclonal antibodies, however, only bind to one epitope of the target antigen. They are typically produced by fusion of the antibody-producing cells in an immunized animals spleen. Tissue culture supernat can also be used to purify the latter.
The most common method of western blotting uses enzymatic labels. These techniques are very sensitive and require additional steps. Examples of such enzymes include alkaline-phosphatase and horseradish peoxidase. These enzymes are highly sensitive, but have a slower reaction time and lower signal-to–noise ratios. So, what is the best method for detecting protein in a blot?
Cleansing the BAG1 marker can be difficult if you don't follow these simple steps. There are several steps to cleaning the BAG1 mark. First, you need to get rid of any residue. If you're unable to remove the remnant, use an antiseptic. Use a mild solution of water with a few drops dishwashing detergent to clean the marker. Once the residue is gone, test the marker again.
PMID: 8524784 by Zeiner M., et al. A protein that interacts with members of the nuclear hormone receptor family: identification and cDNA cloning.
PMID: 8812483 by Takayama S., et al. Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12.
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