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Product Info Summary
| SKU: | PA1973 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Zonula occludens protein 3/TJP3 Antibody Picoband®
SKU/Catalog Number
PA1973
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zonula occludens protein 3/TJP3 Antibody catalog # PA1973. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zonula occludens protein 3/TJP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1973)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Zonula occludens protein 3, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1973 is reactive to Tjp3 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
99324 MW
Background of TJP3
TJP3 (TIGHT JUNCTION PROTEIN 3), also called ZO3, is a protein that in humans is encoded by the TJP3 gene. TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al., 1999). Haskins et al. (1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds directly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al. (2002) determined that Tjp3 interacted with PATJ (INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1973 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Heart Tissue,
IHC: Rat Intestine Tissue, Mouse Liver Tissue
Validation Images & Assay Conditions
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Anti-Zonula occludens protein 3 antibody, PA1973, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
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Anti-Zonula occludens protein 3 antibody, PA1973, IHC(P)
IHC(P): Rat Intestine Tissue
Click image to see more details
Anti-Zonula occludens protein 3 antibody, PA1973, IHC(P)
IHC(P): Mouse Liver Tissue
Protein Target Info & Infographic
Gene/Protein Information For Tjp3 (Source: Uniprot.org, NCBI)
Gene Name
Tjp3
Full Name
Tight junction protein ZO-3
Weight
99324 MW
Superfamily
MAGUK family
Alternative Names
Tight junction protein ZO-3;Tight junction protein 3;Zona occludens protein 3;Zonula occludens protein 3;Tjp3;Zo3; TJP3 ZO-3, ZO3 tight junction protein 3 tight junction protein ZO-3|tight junction protein 3 (zona occludens 3)|zona occludens protein 3|zonula occludens protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tjp3, check out the Tjp3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tjp3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Zonula occludens protein 3/TJP3 Antibody Picoband® (PA1973)
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No publications found for PA1973
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Zonula occludens protein 3/TJP3 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Zonula occludens protein 3/TJP3 antibody PA1973. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
I see that the anti-Zonula occludens protein 3/TJP3 antibody PA1973 works with IHC, what is the protocol used to produce the result images on the product page?
A. Patel
Verified customer
Asked: 2019-01-22
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-22
Question
We are currently using anti-Zonula occludens protein 3/TJP3 antibody PA1973 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
The anti-Zonula occludens protein 3/TJP3 antibody (PA1973) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-15
Question
I would like to test anti-Zonula occludens protein 3/TJP3 antibody PA1973 on rat liver for research purposes, then I may be interested in using anti-Zonula occludens protein 3/TJP3 antibody PA1973 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
The products we sell, including anti-Zonula occludens protein 3/TJP3 antibody PA1973, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-16
Question
Is there a BSA free version of anti-Zonula occludens protein 3/TJP3 antibody PA1973 available?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Zonula occludens protein 3/TJP3 antibody PA1973 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-05
Question
Does anti-Zonula occludens protein 3/TJP3 antibody PA1973 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
According to the expression profile of liver, TJP3 is highly expressed in liver. So, it is likely that anti-Zonula occludens protein 3/TJP3 antibody PA1973 will work for IHC with liver.
Boster Scientific Support
Answered: 2017-05-25
Question
My question regarding product PA1973, anti-Zonula occludens protein 3/TJP3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Miller
Verified customer
Asked: 2014-04-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1973 anti-Zonula occludens protein 3/TJP3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-04-10
