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Product Info Summary
| SKU: | A30772 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-ZNF295 ZBTB21 Antibody
SKU/Catalog Number
A30772
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ZNF295 ZBTB21 Antibody (Catalog# A30772). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZNF295 ZBTB21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30772)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ZNF295. Immunogen sequence location: 731 - 780
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30772 is reactive to ZBTB21 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
118870 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30772 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ZNF295 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For ZBTB21 (Source: Uniprot.org, NCBI)
Gene Name
ZBTB21
Full Name
Zinc finger and BTB domain-containing protein 21
Weight
118870 MW
Alternative Names
ZNF295;KIAA1227;ZBTB21;Zinc finger protein 295;Zinc finger and BTB domain-containing protein 21 ZBTB21 ZNF295 zinc finger and BTB domain containing 21 zinc finger and BTB domain-containing protein 21|zinc finger protein 295
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZBTB21, check out the ZBTB21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZBTB21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZNF295 ZBTB21 Antibody (A30772)
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No publications found for A30772
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-ZNF295 ZBTB21 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ZNF295 antibody A30772. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-12
Question
Will A30772 anti-ZNF295 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
As indicated on the product datasheet, A30772 anti-ZNF295 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-19
Question
I was wanting to use your anti-ZNF295 antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
It shows on the product datasheet, A30772 anti-ZNF295 antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
We are currently using anti-ZNF295 antibody A30772 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
G. Moore
Verified customer
Asked: 2019-01-24
Answer
The anti-ZNF295 antibody (A30772) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-24
Question
My question regarding product A30772, anti-ZNF295 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30772 anti-ZNF295 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-30
Question
Does anti-ZNF295 antibody A30772 work for IHC with liver?
P. Li
Verified customer
Asked: 2015-08-14
Answer
According to the expression profile of liver, ZBTB21 is highly expressed in liver. So, it is likely that anti-ZNF295 antibody A30772 will work for IHC with liver.
Boster Scientific Support
Answered: 2015-08-14
Question
Would anti-ZNF295 antibody A30772 work on dog IHC with placenta?
J. Parker
Verified customer
Asked: 2015-01-27
Answer
Our lab technicians have not validated anti-ZNF295 antibody A30772 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ZNF295 antibody A30772 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog placenta in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-27
