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Product Info Summary
| SKU: | A04050-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ZIC3 Antibody Picoband®
SKU/Catalog Number
A04050-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZIC3 Antibody Picoband® catalog # A04050-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZIC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04050-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ZIC3 recombinant protein (Position: N113-N455).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04050-2 is reactive to ZIC3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56-60 kDa
Calculated molecular weight
23310 MW
Background of ZIC3
ZIC3 is a member of the Zinc finger of the cerebellum (ZIC) protein family. This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04050-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ZIC3 using anti-ZIC3 antibody (A04050-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC3 antigen affinity purified polyclonal antibody (Catalog # A04050-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIC3 at approximately 56-60KD. The expected band size for ZIC3 is at 56-60KD.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-ZIC3 antibody (A04050-2).
Overlay histogram showing MCF-7 cells stained with A04050-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZIC3 Antibody (A04050-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ZIC3 (Source: Uniprot.org, NCBI)
Gene Name
ZIC3
Full Name
Zinc finger protein ZIC 3
Weight
23310 MW
Superfamily
GLI C2H2-type zinc-finger protein family
Alternative Names
Microtubule-associated tumor suppressor 1; AT2 receptor-binding protein; Angiotensin-II type 2 receptor-interacting protein; Mitochondrial tumor suppressor 1; MTUS1; ATBP; ATIP; GK1; KIAA1288; MTSG1 ZIC3 HTX, HTX1, VACTERLX, ZNF203 Zic family member 3 zinc finger protein ZIC 3|Zic family member 3 (odd-paired homolog, Drosophila)|heterotaxy 1|zinc finger protein 203|zinc finger protein of the cerebellum 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZIC3, check out the ZIC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZIC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZIC3 Antibody Picoband® (A04050-2)
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